Detailed view for TGME49_254750

Basic information

TDR Targets ID: 259239
Toxoplasma gondii, c3orf15 protein
Source Database / ID:  ToxoDB 

pI: 8.5791 | Length (AA): 586 | MW (Da): 67168 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF14738   Solute carrier (proton/amino acid symporter), TRAMD3 or PAT1

Gene Ontology

Mouse over links to read term descriptions.
GO:0003779   actin binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
248 467 5cwp (A) 18 222 26.00 0.32 0.99 0.746227 -1.02
340 526 5cwm (A) 3 220 34.00 0.058 0.8 0.408913 0.2
457 580 5j0l (A) 4 130 9.00 0.83 0.03 0.363804 -1.36

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 merozoite. Hehl AB
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile VEG Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_130150)

Species Accession Gene Product
Drosophila melanogaster Dmel_CG15143   CG15143 gene product from transcript CG15143-RA
Drosophila melanogaster Dmel_CG15145   CG15145 gene product from transcript CG15145-RA
Drosophila melanogaster Dmel_CG15144   CG15144 gene product from transcript CG15144-RB
Echinococcus granulosus EgrG_000095200   AMY 1 associating protein expressed in testis
Echinococcus multilocularis EmuJ_000278200   hypothetical protein
Echinococcus multilocularis EmuJ_000095200   AMY 1 associating protein expressed in testis
Giardia lamblia GL50803_89828   Hypothetical protein
Homo sapiens ENSG00000183833   MYCBP-associated, testis expressed 1
Leishmania braziliensis LbrM.33.2510   hypothetical protein, conserved
Leishmania donovani LdBPK_332350.1   Solute carrier (proton/amino acid symporter), TRAMD3 or PAT1, putative
Leishmania infantum LinJ.33.2350   hypothetical protein, conserved
Leishmania major LmjF.33.2220   hypothetical protein, conserved
Leishmania mexicana LmxM.32.2220   hypothetical protein, conserved
Mus musculus ENSMUSG00000022805   MYCBP-associated, testis expressed 1
Neospora caninum NCLIV_009010   hypothetical protein, conserved
Plasmodium berghei PBANKA_1420700   MAATS1 domain-containing protein, putative
Plasmodium falciparum PF3D7_0714100   MAATS1 domain-containing protein, putative
Plasmodium knowlesi PKNH_1423000   MAATS1 domain-containing protein, putative
Plasmodium vivax PVX_122935   hypothetical protein, conserved
Plasmodium yoelii PY02617   hypothetical protein
Schistosoma japonicum Sjp_0103480   AMY-1-associating protein expressed in testis 1, putative
Schistosoma japonicum Sjp_0078000   AMY-1-associating protein expressed in testis 1, putative
Schistosoma mansoni Smp_084650   hypothetical protein
Schistosoma mansoni Smp_168020   hypothetical protein
Schistosoma mansoni Smp_168010   hypothetical protein
Schmidtea mediterranea mk4.012243.00  
Schmidtea mediterranea mk4.007906.04  
Schmidtea mediterranea mk4.000946.06  
Trypanosoma brucei gambiense Tbg972.11.3090   hypothetical protein, conserved
Trypanosoma brucei Tb927.11.2790   Solute carrier (proton/amino acid symporter), TRAMD3 or PAT1, putative
Trypanosoma congolense TcIL3000_0_14790   Solute carrier (proton/amino acid symporter), TRAMD3 or PAT1, putative
Trypanosoma congolense TcIL3000.11.2530   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.506303.130   Solute carrier (proton/amino acid symporter), TRAMD3 or PAT1, putative
Trypanosoma cruzi TcCLB.506811.100   Solute carrier (proton/amino acid symporter), TRAMD3 or PAT1, putative
Toxoplasma gondii TGME49_254750   c3orf15 protein
Trichomonas vaginalis TVAG_140020   conserved hypothetical protein

Essentiality

TGME49_254750 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.02.0354 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.02.0354 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.02.0354 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.02.0354 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_254750 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_254750 (Toxoplasma gondii), c3orf15 protein
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