Detailed view for TGME49_226910

Basic information

TDR Targets ID: 259322
Toxoplasma gondii, Amylo-alpha-1,6-glucosidase
Source Database / ID:  ToxoDB 

pI: 6.5574 | Length (AA): 1882 | MW (Da): 208019 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF06202   Amylo-alpha-1,6-glucosidase
PF14701   Glycogen debranching enzyme, glucanotransferase domain
PF14702   Central domain of human glycogen debranching enzyme
PF16561   Glycogen recognition site of AMP-activated protein kinase

Gene Ontology

Mouse over links to read term descriptions.
GO:0004135   amylo-alpha-1,6-glucosidase activity  
GO:0004134   4-alpha-glucanotransferase activity  
GO:0003824   catalytic activity  
GO:0043169   cation binding  
GO:0005978   glycogen biosynthetic process  
GO:0005975   carbohydrate metabolic process  
GO:0005980   glycogen catabolic process  

Metabolic Pathways

Starch and sucrose metabolism (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
52 687 2bhu (A) 33 596 13.00 0 1 -0.08 0.84
352 425 1bpl (B) 199 266 7.00 0.000017 0.01 0.02 -0.1
868 970 2f15 (A) 75 163 24.00 0 1 0.19 0.07
1136 1880 1lf6 (A) 11 680 10.00 0 0.97 -0.05 0.5
23 1860 5d06 (A) 12 1523 34.00 0 1 1.02362 0.97
150 1859 5d06 (A) 129 1522 40.00 0 1 1.03621 0.76
871 960 2qlv (B) 165 243 32.00 0 1 0.373421 -1.17

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 merozoite, ME49 Oocyst. Hehl AB Fritz HM
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_128837)

Species Accession Gene Product
Brugia malayi Bm1_19030   Amylo-alpha-1,6-glucosidase family protein
Brugia malayi Bm1_53305   hypothetical protein
Candida albicans CaO19.8363   similar to S. cerevisiae GDB1 (YPR184W) glycogen debranching enzyme
Candida albicans CaO19.744   similar to S. cerevisiae GDB1 (YPR184W) glycogen debranching enzyme
Caenorhabditis elegans CELE_R06A4.8   Protein AGL-1
Cryptosporidium hominis Chro.60114   glycine-rich protein 2 (GRP2)
Cryptosporidium parvum cgd6_880   Gdb1p; glycogen debranching enzyme
Dictyostelium discoideum DDB_G0287569   glycogen debranching enzyme
Drosophila melanogaster Dmel_CG9485   CG9485 gene product from transcript CG9485-RF
Echinococcus granulosus EgrG_000644500   glycogen debranching enzyme
Entamoeba histolytica EHI_098360   glycogen debranching enzyme, putative
Echinococcus multilocularis EmuJ_000644500   glycogen debranching enzyme
Giardia lamblia GL50803_10885   4-alpha-glucanotransferase, amylo-alpha-1,6-glucosidase
Homo sapiens ENSG00000162688   amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase
Loa Loa (eye worm) LOAG_11769   amylo-alpha-1,6-glucosidase
Loa Loa (eye worm) LOAG_07652   hypothetical protein
Mus musculus ENSMUSG00000033400   amylo-1,6-glucosidase, 4-alpha-glucanotransferase
Neospora caninum NCLIV_046100   glycogen debranching enzyme, putative
Saccharomyces cerevisiae YPR184W   bifunctional 4-alpha-glucanotransferase/amylo-alpha-1,6-glucosidase
Schistosoma japonicum Sjp_0097780   ko:K00705 4-alpha-glucanotransferase [EC2.4.1.25], putative
Schistosoma japonicum Sjp_0209490   Glycogen debranching enzyme, putative
Schistosoma japonicum Sjp_0209480   Glycogen debranching enzyme, putative
Schistosoma japonicum Sjp_0209500   Glycogen debranching enzyme, putative
Schistosoma mansoni Smp_007650   hypothetical protein
Schistosoma mansoni Smp_007640   hypothetical protein
Schmidtea mediterranea mk4.006507.01   Glycogen debranching enzyme
Schmidtea mediterranea mk4.006507.00   Glycogen debranching enzyme
Toxoplasma gondii TGME49_226910   Amylo-alpha-1,6-glucosidase
Trichomonas vaginalis TVAG_143490   glycogen debranching enzyme, putative
Trichomonas vaginalis TVAG_330630   glycogen debranching enzyme, putative

Essentiality

TGME49_226910 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
CELE_R06A4.8 Caenorhabditis elegans embryonic lethal wormbase
TGME49_226910 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase Compounds References
Oryctolagus cuniculus Glycogen debranching enzyme Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0409 0.3664 1
0.0405 0.3013 1
0.013 0.2666 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier TGME49_226910 (Toxoplasma gondii), Amylo-alpha-1,6-glucosidase
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