Detailed view for TGME49_244240

Basic information

TDR Targets ID: 259367
Toxoplasma gondii, hypothetical protein
Source Database / ID:  ToxoDB 

pI: 8.2614 | Length (AA): 438 | MW (Da): 47936 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
GO:0008168   methyltransferase activity  
GO:0003676   nucleic acid binding  
GO:0032259   methylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
331 437 3q87 (B) 71 163 40.00 0 1 0.656892 -0.85

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Hehl AB Fritz HM Sibley/Greg
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_127713)

Species Accession Gene Product
Arabidopsis thaliana AT3G13440   S-adenosyl-L-methionine-dependent methyltransferase-like protein
Babesia bovis BBOV_II004490   conserved hypothetical protein
Brugia malayi Bm1_54730   methylase family protein
Candida albicans CaO19.331   protein methylation
Candida albicans CaO19.7963   protein methylation
Caenorhabditis elegans CELE_C33C12.9   Protein C33C12.9
Cryptosporidium hominis Chro.40428   hypothetical protein
Cryptosporidium parvum cgd4_3740   Ydr140wp-like HemK family methylase. archaeal-like. RNA methylase
Dictyostelium discoideum DDB_G0292048   hypothetical protein
Drosophila melanogaster Dmel_CG9960   CG9960 gene product from transcript CG9960-RA
Echinococcus granulosus EgrG_001192400   hemK methyltransferase family
Entamoeba histolytica EHI_121470   DNA methyltransferase, putative
Echinococcus multilocularis EmuJ_001192400   hemK methyltransferase family
Giardia lamblia GL50803_1903   DNA methyltransferase
Homo sapiens 29104   N-6 adenine-specific DNA methyltransferase 1 (putative)
Leishmania braziliensis LbrM.32.2300   hypothetical protein, conserved
Leishmania donovani LdBPK_322220.1   hypothetical protein, conserved
Leishmania infantum LinJ.32.2220   hypothetical protein, conserved
Leishmania major LmjF.32.2090   hypothetical protein, conserved
Leishmania mexicana LmxM.31.2090   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_02109   methylase
Mus musculus ENSMUSG00000044442   N-6 adenine-specific DNA methyltransferase 1 (putative)
Oryza sativa 4334624   Os03g0824200
Onchocerca volvulus OVOC10219   HemK methyltransferase family member 2 homolog
Plasmodium berghei PBANKA_1401600   methyltransferase-like protein, putative
Plasmodium falciparum PF3D7_1303100   methyltransferase-like protein, putative
Plasmodium knowlesi PKNH_1403300   methyltransferase-like protein, putative
Plasmodium vivax PVX_121995   Putative N6-DNA-methyltransferase, putative
Plasmodium yoelii PY03902   hypothetical protein
Saccharomyces cerevisiae YDR140W   Mtq2p
Schistosoma japonicum Sjp_0016240   ko:K00571 site-specific DNA-methyltransferase (adenine-specific) [EC2.1.1.72], putative
Schistosoma mansoni Smp_024800   n6-DNA-methyltransferase
Schmidtea mediterranea mk4.001089.10   HemK methyltransferase family member 2
Trypanosoma brucei gambiense Tbg972.11.17170   hypothetical protein, conserved
Trypanosoma brucei Tb927.11.15290   eRF1 methyltransferase catalytic subunit, putative
Trypanosoma congolense TcIL3000.11.15430   eRF1 methyltransferase catalytic subunit, putative
Trypanosoma cruzi TcCLB.511711.30   eRF1 methyltransferase catalytic subunit, putative
Trypanosoma cruzi TcCLB.506425.120   eRF1 methyltransferase catalytic subunit, putative
Toxoplasma gondii TGME49_244240   hypothetical protein
Theileria parva TP04_0566   DNA-methyltransferase, putative
Trichomonas vaginalis TVAG_102430   n6-DNA-methyltransferase, putative

Essentiality

TGME49_244240 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.6940 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.6940 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.6940 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.6940 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1401600 Plasmodium berghei Slow plasmo
TGME49_244240 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

2 literature references were collected for this gene.

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User comments

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Gene identifier TGME49_244240 (Toxoplasma gondii), hypothetical protein
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