Detailed view for TGME49_293060

Basic information

TDR Targets ID: 259473
Toxoplasma gondii, SPRY domain-containing protein
Source Database / ID:  ToxoDB 

pI: 5.2256 | Length (AA): 740 | MW (Da): 80291 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00622   SPRY domain
PF10607   CTLH/CRA C-terminal to LisH motif domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
41 249 5jia (A) 35 233 27.00 0.0000053 1 0.599632 -0.25
170 205 4p9j (C) 1134 1169 47.00 0.8 0.57 0.363349 2.05
302 373 5hmo (A) 815 898 43.00 0.03 0.01 0.538997 -1.78

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 Oocyst. Fritz HM
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile VEG Tachyzoite, ME49 merozoite. Gregory Hehl AB
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_128341)

Species Accession Gene Product
Arabidopsis thaliana AT4G09340   SPla/RYanodine receptor (SPRY) domain-containing protein
Arabidopsis thaliana AT1G35470   RanBPM-like protein
Babesia bovis BBOV_IV010250   PRY domain containing protein
Brugia malayi Bm1_02375   hypothetical protein
Brugia malayi Bm1_02380   SPRY domain containing protein
Caenorhabditis elegans CELE_Y54E5A.7   Protein Y54E5A.7, isoform D
Dictyostelium discoideum DDB_G0292780   hypothetical protein
Drosophila melanogaster Dmel_CG42236   Ran-binding protein M
Echinococcus granulosus EgrG_000657210   ran binding protein 9
Echinococcus granulosus EgrG_000467400   ran binding protein 9
Entamoeba histolytica EHI_091560   hypothetical protein, conserved domain containing
Echinococcus multilocularis EmuJ_000467400   ran binding protein 9
Echinococcus multilocularis EmuJ_000657210   ran binding protein 9
Homo sapiens ENSG00000141084   RAN binding protein 10
Homo sapiens ENSG00000010017   RAN binding protein 9
Loa Loa (eye worm) LOAG_03825   hypothetical protein
Mus musculus ENSMUSG00000037415   RAN binding protein 10
Mus musculus ENSMUSG00000038546   RAN binding protein 9
Neospora caninum NCLIV_000110   Arabinogalactan protein, related
Plasmodium berghei PBANKA_1212700   conserved Plasmodium protein, unknown function
Plasmodium falciparum PF3D7_1014300   conserved protein, unknown function
Plasmodium knowlesi PKNH_0814400   conserved Plasmodium protein, unknown function
Plasmodium vivax PVX_094930   hypothetical protein, conserved
Plasmodium yoelii PY04751   arabinogalactan protein
Plasmodium yoelii PY04750   SPRY domain, putative
Schistosoma japonicum Sjp_0029280   IPR006595,CTLH, C-terminal to LisH motif;IPR013144,CT11-RanBPM,domain-containing
Schistosoma japonicum Sjp_0311550   similar to Uncharacterized protein C1259.12c, putative
Schistosoma japonicum Sjp_0117750   hypothetical protein
Schistosoma mansoni Smp_161080   ran binding protein 9-related
Schmidtea mediterranea mk4.002346.03  
Toxoplasma gondii TGME49_293060   SPRY domain-containing protein
Theileria parva TP01_0782   hypothetical protein

Essentiality

TGME49_293060 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
TGME49_293060 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TGME49_293060 (Toxoplasma gondii), SPRY domain-containing protein
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