Detailed view for TGME49_262750

Basic information

TDR Targets ID: 259475
Toxoplasma gondii, histone lysine methyltransferase, SET, putative
Source Database / ID:  ToxoDB 

pI: 5.3317 | Length (AA): 521 | MW (Da): 53633 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00856   SET domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
71 146 2qtw (B) 585 675 14.00 0 0.03 0.306573 -0.42
428 505 3n71 (A) 196 281 35.00 0.00051 1 0.441712 0.46
431 476 5f5e (A) 3896 3944 41.00 0.84 0.27 0.374292 0.29
437 490 3f9x (A) 298 350 32.00 0 0.84 0.435347 0.29

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 merozoite, ME49 Oocyst, ME49 Bradyzoite. Hehl AB Fritz HM Sibley/Greg
Show/Hide expression data references
  • Gregory ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_138304)

Species Accession Gene Product
Cryptosporidium hominis Chro.10248   hypothetical protein
Cryptosporidium parvum cgd1_2170   SET domain containing protein with a cysteine cluster at the C-terminus
Dictyostelium discoideum DDB_G0292140   SET domain-containing protein
Leishmania braziliensis LbrM.23.0910   hypothetical protein, conserved
Leishmania donovani LdBPK_230990.1   SET domain containing protein, putative
Leishmania infantum LinJ.23.0990   hypothetical protein, conserved
Leishmania major LmjF.23.0820   hypothetical protein, conserved
Leishmania mexicana LmxM.23.0820   hypothetical protein, conserved
Neospora caninum NCLIV_025110   hypothetical protein, conserved
Trypanosoma brucei gambiense Tbg972.8.2230   hypothetical protein, conserved
Trypanosoma brucei Tb927.8.2690   SET domain containing protein, putative
Trypanosoma congolense TcIL3000_8_2550   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.504151.69   SET domain containing protein, putative
Trypanosoma cruzi TcCLB.509459.70   SET domain containing protein, putative
Toxoplasma gondii TGME49_262750   histone lysine methyltransferase, SET, putative

Essentiality

TGME49_262750 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.2690 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.2690 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.2690 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.2690 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_262750 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier TGME49_262750 (Toxoplasma gondii), histone lysine methyltransferase, SET, putative
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