Detailed view for TGME49_289570

Basic information

TDR Targets ID: 259509
Toxoplasma gondii, phosphatidylinositol transfer protein
Source Database / ID:  ToxoDB 

pI: 6.3535 | Length (AA): 1912 | MW (Da): 209610 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 4

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00169   PH domain
PF01237   Oxysterol-binding protein
PF02121   Phosphatidylinositol transfer protein

Gene Ontology

Mouse over links to read term descriptions.
GO:0005548   phospholipid transporter activity  
GO:0005622   intracellular  
GO:0015914   phospholipid transport  
GO:0008202   steroid metabolic process  
GO:0006810   transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 10 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 337 1t27 (A) 2 263 34.00 0 1 0.206955 0.91
1 335 2a1l (A) 2 260 36.00 0 1 0.224809 0.63
462 579 2yry (A) 1 120 23.00 0 1 0.358315 -0.28
472 589 2d9x (A) 3 114 28.00 0 0.99 0.352315 -0.69
475 579 1upq (A) 48 153 26.00 0 0.99 0.474516 -1.17
1074 1226 3e8u (H) 44 181 31.00 0.54 0.25 -0.0516791 2.67
1348 1570 5cwp (A) 6 229 12.00 0.65 0.03 0.329332 -1.08
1594 1908 4ic4 (A) 642 964 23.00 0 1 0.328449 0.28
1602 1898 1zhx (A) 21 353 22.00 0 1 0.316935 -0.19
1664 1892 3spw (A) 98 347 28.00 0.0038 0.21 0.0440699 1.37

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 merozoite. Hehl AB
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_136385)

Species Accession Gene Product
Dictyostelium discoideum DDB_G0284353   oxysterol binding family protein, member 9
Giardia lamblia GL50803_14833   Hypothetical protein
Neospora caninum NCLIV_041820   hypothetical protein
Toxoplasma gondii TGME49_289570   phosphatidylinositol transfer protein
Trichomonas vaginalis TVAG_335580   oxysterol-binding protein 1A, putative
Trichomonas vaginalis TVAG_512110   oxysterol-binding protein, putative
Trichomonas vaginalis TVAG_026290   oxysterol-binding protein, putative
Trichomonas vaginalis TVAG_409990   ergosterol biosynthesis protein, putative

Essentiality

TGME49_289570 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
TGME49_289570 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_289570 (Toxoplasma gondii), phosphatidylinositol transfer protein
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