Detailed view for TGME49_221940

Basic information

TDR Targets ID: 259631
Toxoplasma gondii, adaptin n terminal region domain-containing protein
Source Database / ID:  ToxoDB 

pI: 8.4175 | Length (AA): 1348 | MW (Da): 147534 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01602   Adaptin N terminal region

Gene Ontology

Mouse over links to read term descriptions.
GO:0030117   membrane coat  
GO:0005515   protein binding  
GO:0005488   binding  
GO:0016192   vesicle-mediated transport  
GO:0006886   intracellular protein transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 13 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
516 1314 2bpt (A) 2 849 9.00 0 1 0.22 0.03
754 1408 1wa5 (C) 2 777 10.00 0 0.72 0.09 0.53
784 1242 2bpt (A) 315 793 11.00 0 0.93 0.44 -0.53
2 780 2jkr (A) 7 615 31.00 0 1 0.594893 0.6
3 769 4p6z (G) 2 587 23.00 0 1 0.469991 0.76
5 398 4nee (G) 10 392 26.00 0 1 0.637885 -0.39
5 512 4nee (G) 10 396 36.00 0 1 0.448055 0.52
76 756 1u6g (C) 54 845 12.00 0 0.99 0.512193 0.79
435 1254 2q5d (B) 60 873 10.00 0.071 0.09 0.470909 1.13
451 634 3woz (A) 651 844 11.00 0 0.88 0.266499 -0.53
875 957 5d2s (A) 100 183 14.00 0 0.01 0.419573 -2.62
890 963 5hmo (A) 819 893 30.00 0.047 0.03 0.544096 -2.03
1073 1251 1b9k (A) 785 934 29.00 0.74 0.66 0.0919893 1.1

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Oocyst. Fritz HM
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_127910)

Species Accession Gene Product
Arabidopsis thaliana AT5G22780   AP-2 complex subunit alpha-2
Arabidopsis thaliana AT5G22770   alpha-adaptin
Babesia bovis BBOV_I001880   conserved hypothetical protein
Brugia malayi Bm1_07200   Alpha-adaptin homolog
Candida albicans CaO19.2786   similar to S. cerevisiae APL3 (YBL037W) Alpha-adaptin, subunit of the clathrin-associated protein AP-2 complex
Candida albicans CaO19.10302   similar to S. cerevisiae APL3 (YBL037W) Alpha-adaptin, subunit of the clathrin-associated protein AP-2 complex
Caenorhabditis elegans CELE_T20B5.1   Protein APA-2
Dictyostelium discoideum DDB_G0273501   adaptor-related protein complex 2, alpha subunit
Dictyostelium discoideum DDB_G0273439   adaptor-related protein complex 2, alpha subunit
Drosophila melanogaster Dmel_CG4260   Adaptor Protein complex 2, alpha subunit
Echinococcus granulosus EgrG_001050200   AP 2 complex subunit alpha 2
Entamoeba histolytica EHI_045710   AP-2 complex protein, putative
Echinococcus multilocularis EmuJ_001050200   AP 2 complex subunit alpha 2
Giardia lamblia GL50803_17304   Alpha adaptin
Homo sapiens ENSG00000183020   adaptor-related protein complex 2, alpha 2 subunit
Homo sapiens ENSG00000196961   adaptor-related protein complex 2, alpha 1 subunit
Leishmania braziliensis LbrM.07.0050   alpha-adaptin-like protein
Leishmania donovani LdBPK_070060.1   alpha-adaptin-like protein
Leishmania infantum LinJ.07.0060   alpha-adaptin-like protein
Leishmania major LmjF.07.0050   alpha-adaptin-like protein
Leishmania mexicana LmxM.07.0050   alpha-adaptin-like protein
Loa Loa (eye worm) LOAG_05403   adaptor protein complex AP-2
Mus musculus ENSMUSG00000060279   adaptor-related protein complex 2, alpha 1 subunit
Mus musculus ENSMUSG00000002957   adaptor-related protein complex 2, alpha 2 subunit
Neospora caninum NCLIV_005350   hypothetical protein
Saccharomyces cerevisiae YBL037W   Apl3p
Schistosoma japonicum Sjp_0080950   AP-2 complex subunit alpha-2, putative
Schistosoma mansoni Smp_074020   alpha adaptin carboxyl-terminal domain
Schmidtea mediterranea mk4.005112.01  
Schmidtea mediterranea mk4.003684.00   AP-2 complex subunit alpha
Schmidtea mediterranea mk4.003684.01   AP-2 complex subunit alpha
Schmidtea mediterranea mk4.005112.00   AP-2 complex subunit alpha
Trypanosoma cruzi TcCLB.511391.140   alpha-adaptin-like, putative
Toxoplasma gondii TGME49_221940   adaptin n terminal region domain-containing protein
Theileria parva TP03_0146   hypothetical protein
Trichomonas vaginalis TVAG_182130   conserved hypothetical protein
Trichomonas vaginalis TVAG_547230   adaptin, alpha/gamma/epsilon, putative
Trichomonas vaginalis TVAG_010930   adaptin, alpha/gamma/epsilon, putative
Trichomonas vaginalis TVAG_010940   adaptin, alpha/gamma/epsilon, putative
Trichomonas vaginalis TVAG_486360   adaptin, alpha/gamma/epsilon, putative
Trichomonas vaginalis TVAG_213470   conserved hypothetical protein

Essentiality

TGME49_221940 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
CELE_T20B5.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_T20B5.1 Caenorhabditis elegans slow growth wormbase
TGME49_221940 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_221940 (Toxoplasma gondii), adaptin n terminal region domain-containing protein
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