Detailed view for TGME49_313470

Basic information

TDR Targets ID: 259668
Toxoplasma gondii, helix-hairpin-helix motif domain-containing protein
Source Database / ID:  ToxoDB 

pI: 8.191 | Length (AA): 860 | MW (Da): 93892 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00730   HhH-GPD superfamily base excision DNA repair protein

Gene Ontology

Mouse over links to read term descriptions.
GO:0004519   endonuclease activity  
GO:0003824   catalytic activity  
GO:0006284   base-excision repair  
GO:0006281   DNA repair  

Metabolic Pathways

Base excision repair (KEGG)

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
181 605 1rrq (A) 9 360 28.00 0 1 0.56 0.72
207 311 1ngn (A) 429 527 19.00 0.0000000057 0.74 0.47 -1.31
246 458 1kg2 (A) 12 224 35.00 0.000000000036 1 0.770074 -0.91
248 599 5dpk (A) 20 358 28.00 0.000000000006 1 0.698702 0.22
251 443 1kg2 (A) 15 209 42.00 0 1 0.756319 -0.61
263 442 1kea (A) 34 213 28.00 0 1 0.564302 -0.53

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 merozoite, ME49 Oocyst, ME49 Bradyzoite. Hehl AB Fritz HM Sibley/Greg
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_128159)

Species Accession Gene Product
Arabidopsis thaliana AT4G12740   HhH-GPD base excision DNA repair family protein
Chlamydia trachomatis CT_107   A/G-specific adenine glycosylase
Dictyostelium discoideum DDB_G0270764   A/G-specific adenine DNA glycosylase
Escherichia coli b2961   adenine DNA glycosylase
Entamoeba histolytica EHI_010700   A/G-specific adenine glycosylase, putative
Homo sapiens ENSG00000132781   mutY homolog
Leishmania braziliensis LbrM.28.2340   A/G-specific adenine glycosylase, putative
Leishmania donovani LdBPK_282290.1   A/G-specific adenine glycosylase, putative
Leishmania infantum LinJ.28.2290   A/G-specific adenine glycosylase, putative
Leishmania major LmjF.28.2140   A/G-specific adenine glycosylase, putative
Leishmania mexicana LmxM.28.2140   A/G-specific adenine glycosylase, putative
Mycobacterium leprae ML1920   PROBABLE ADENINE GLYCOSYLASE MUTY
Mus musculus ENSMUSG00000028687   mutY homolog (E. coli)
Mycobacterium tuberculosis Rv3589   Probable adenine glycosylase MutY
Mycobacterium ulcerans MUL_4165   adenine glycosylase MutY
Neospora caninum NCLIV_056760   helix-hairpin-helix motif-containing protein, putative
Oryza sativa 4351781   Os12g0211400
Plasmodium berghei PBANKA_0918700   A/G-specific adenine glycosylase, putative
Plasmodium falciparum PF3D7_1129500   A/G-specific adenine glycosylase, putative
Plasmodium knowlesi PKNH_0927500   A/G-specific adenine glycosylase, putative
Plasmodium vivax PVX_092090   A/G-specific adenine glycosylase, putative
Plasmodium yoelii PY05666   A/G-specific adenine glycosylase, putative
Schistosoma japonicum Sjp_0031390   ko:K03575 A/G-specific adenine glycosylase, putative
Schistosoma mansoni Smp_001020   DNA glycosylase
Trypanosoma brucei gambiense Tbg972.11.12830   A/G-specific adenine glycosylase, putative
Trypanosoma brucei Tb927.11.11440   A/G-specific adenine glycosylase, putative
Trypanosoma congolense TcIL3000_0_22020   A/G-specific adenine glycosylase, putative
Trypanosoma cruzi TcCLB.511803.20   A/G-specific adenine glycosylase, putative
Toxoplasma gondii TGME49_313470   helix-hairpin-helix motif domain-containing protein
Treponema pallidum TP0343   A/G-specific adenine glycosylase

Essentiality

TGME49_313470 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu3651 Mycobacterium tuberculosis non-essential nmpdr
Tb11.01.3270 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.3270 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.3270 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.01.3270 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b2961 Escherichia coli non-essential goodall
PBANKA_0918700 Plasmodium berghei Dispensable plasmo
TGME49_313470 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_313470 (Toxoplasma gondii), helix-hairpin-helix motif domain-containing protein
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