Detailed view for TGME49_310760

Basic information

TDR Targets ID: 259669
Toxoplasma gondii, protein phosphatase 2C domain-containing protein
Source Database / ID:  ToxoDB 

pI: 7.3141 | Length (AA): 344 | MW (Da): 37632 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00481   Protein phosphatase 2C

Gene Ontology

Mouse over links to read term descriptions.
GO:0004722   protein serine/threonine phosphatase activity  
GO:0003824   catalytic activity  
GO:0043169   cation binding  
GO:0006470   protein amino acid dephosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
16 341 2p8e (A) 5 295 26.00 0.0000000033 1 1.03047 0.23
41 344 3kdj (B) 128 425 25.00 0.000000029 1 1.10452 -0.25
44 279 2iq1 (A) 97 299 38.00 0 1 0.716147 1.01
48 344 3rt0 (A) 196 506 30.00 0 1 1.11947 0.15
48 342 2pnq (A) 46 455 23.00 0 1 0.880958 -0.17

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Hehl AB Fritz HM Sibley/Greg
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_131254)

Species Accession Gene Product
Arabidopsis thaliana AT1G18030   putative protein phosphatase 2C 8
Babesia bovis BBOV_IV000460   protein phosphatase 2C, putative
Brugia malayi Bm1_14595   Protein phosphatase 2C containing protein
Caenorhabditis elegans CELE_F33A8.6   Protein F33A8.6
Echinococcus granulosus EgrG_000218900   JmjC domain containing protein 4
Echinococcus granulosus EgrG_000218400   integrin linked kinase associated
Entamoeba histolytica EHI_086040   protein phosphatase, putative
Entamoeba histolytica EHI_179330   protein phosphatase family protein
Echinococcus multilocularis EmuJ_000218400   integrin linked kinase associated
Echinococcus multilocularis EmuJ_000218900   JmjC domain containing protein 4
Homo sapiens ENSG00000132323   integrin-linked kinase-associated serine/threonine phosphatase
Loa Loa (eye worm) LOAG_09542   protein phosphatase 2C containing protein
Mus musculus ENSMUSG00000026309   integrin-linked kinase-associated serine/threonine phosphatase 2C
Neospora caninum NCLIV_054810   hypothetical protein
Oryza sativa 4346692   Os09g0314400
Plasmodium berghei PBANKA_1427000   protein phosphatase PPM7
Plasmodium falciparum PF3D7_0810500   protein phosphatase PPM7, putative
Plasmodium knowlesi PKNH_1430200   protein phosphatase PPM7, putative
Plasmodium vivax PVX_123245   protein phosphatase PPM7, putative
Plasmodium yoelii PY06112   hypothetical protein
Plasmodium yoelii PY06111   hypothetical protein
Schistosoma japonicum Sjp_0217700   ko:K01090 protein phosphatase [EC3.1.3.16], putative
Schistosoma japonicum Sjp_0217710   JmjC domain-containing protein 4 homolog, putative
Schistosoma mansoni Smp_147870   protein phosphatase 2C
Schmidtea mediterranea mk4.005858.00  
Schmidtea mediterranea mk4.017651.00  
Schmidtea mediterranea mk4.000887.01  
Toxoplasma gondii TGME49_310760   protein phosphatase 2C domain-containing protein
Theileria parva TP01_0064   protein phosphatase 2C, putative

Essentiality

TGME49_310760 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
PBANKA_1427000 Plasmodium berghei Dispensable plasmo
TGME49_310760 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.3

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Calcineurin (3.1.3.16 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

6 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TGME49_310760 (Toxoplasma gondii), protein phosphatase 2C domain-containing protein
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