Detailed view for TGME49_227440

Basic information

TDR Targets ID: 259754
Toxoplasma gondii, WD domain, G-beta repeat-containing protein
Source Database / ID:  ToxoDB 

pI: 5.8715 | Length (AA): 461 | MW (Da): 50567 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00400   WD domain, G-beta repeat

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
15 356 1erj (A) 342 708 18.00 0 1 0.67 0.97
6 304 5kdo (B) 29 314 26.00 0.0000036 1 0.84539 0.3
34 120 5k1a (B) 67 148 30.00 0.029 0.43 0.55152 -0.93
41 141 1p22 (A) 389 479 34.00 0.000066 0.9 0.603889 -0.68
41 127 3k26 (A) 189 274 33.00 0.11 0.73 0.63252 -0.84

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Oocyst. Fritz HM
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile VEG Tachyzoite. Gregory
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_129602)

Species Accession Gene Product
Arabidopsis thaliana AT5G64730   transducin/WD40 domain-containing protein
Babesia bovis BBOV_II003810   WD domain, G-beta repeat containing protein
Brugia malayi Bm1_42780   WD domain, G-beta repeat
Caenorhabditis elegans CELE_F33G12.2   Protein F33G12.2
Dictyostelium discoideum DDB_G0289711   WD40 repeat-containing protein
Drosophila melanogaster Dmel_CG4935   CG4935 gene product from transcript CG4935-RA
Echinococcus granulosus EgrG_001172100   WD40 repeat domain containing protein 83
Entamoeba histolytica EHI_182680   WD domain containing protein
Echinococcus multilocularis EmuJ_001172100   WD40 repeat domain containing protein 83
Homo sapiens ENSG00000123154   WD repeat domain 83
Loa Loa (eye worm) LOAG_07159   hypothetical protein
Loa Loa (eye worm) LOAG_10563   WD domain-containing protein
Mus musculus ENSMUSG00000005150   WD repeat domain containing 83
Oryza sativa 4329605   Os02g0543400
Plasmodium berghei PBANKA_1226900   mitogen-activated protein kinase organizer 1, putative
Plasmodium falciparum PF3D7_0803300   mitogen-activated protein kinase organizer 1, putative
Plasmodium knowlesi PKNH_0115600   mitogen-activated protein kinase organizer 1, putative
Plasmodium vivax PVX_093580   mitogen-activated protein kinase organizer 1, putative
Schistosoma japonicum Sjp_0100270   Mitogen-activated protein kinase organizer 1, putative
Schistosoma mansoni Smp_049890   hypothetical protein
Schmidtea mediterranea mk4.002083.06   WD-repeat protein, putative
Toxoplasma gondii TGME49_227440   WD domain, G-beta repeat-containing protein
Theileria parva TP04_0194   hypothetical protein, conserved
Trichomonas vaginalis TVAG_212710   WD-repeat protein, putative

Essentiality

TGME49_227440 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
TGME49_227440 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_227440 (Toxoplasma gondii), WD domain, G-beta repeat-containing protein
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