Detailed view for TGME49_228690

Basic information

TDR Targets ID: 259785
Toxoplasma gondii, phosphatidylinositol 3- and 4-kinase
Source Database / ID:  ToxoDB 

pI: 9.363 | Length (AA): 6780 | MW (Da): 733567 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00454   Phosphatidylinositol 3- and 4-kinase

Gene Ontology

Mouse over links to read term descriptions.
GO:0000119   mediator complex  
GO:0016455   RNA polymerase II transcription mediator activity  
GO:0016301   kinase activity  
GO:0048015   phosphoinositide-mediated signaling  
GO:0046854   phosphoinositide phosphorylation  
GO:0006357   regulation of transcription from RNA polymerase II promoter  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 16 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2028 2085 1jgg (A) 103 159 12.00 0.0000087 0 0.15 -0.5
2153 2197 1ygh (A) 99 139 24.00 0.000086 0.02 0.06 -0.28
5959 6053 1e7u (A) 921 1017 40.00 0.000000055 1 0.47 -1.17
23 162 2mu3 (A) 35 188 31.00 0.0013 0.01 -0.064351 2.46
1295 1451 2kr0 (A) 117 257 29.00 0.29 0.01 -0.125744 3.06
2579 2643 5hmo (A) 813 880 37.00 0.11 0.01 0.434587 -1.14
2984 3086 2vn4 (A) 377 478 36.00 0.32 0.62 0.108192 1.59
3527 3638 1w9r (A) 2 119 19.00 0.28 0.15 0.178619 -1.41
3615 3861 5cwq (A) 4 222 23.00 0.0042 0.89 0.215531 -0.24
3624 3844 5cwm (A) 2 227 23.00 0.64 0.99 0.262696 -0.65
5683 5803 5hmo (A) 814 926 13.00 0.0018 0 0.111947 -0.58
5744 6517 1e8y (A) 314 1090 17.00 0 1 0.0592593 1.11
5949 6774 1e8y (A) 480 1090 23.00 0 1 -0.445371 1.65
6568 6773 4jps (A) 849 1060 28.00 0 1 0.273183 -0.36
6574 6761 4uwh (A) 684 868 28.00 0 1 0.344529 -0.88
6604 6759 5t8i (A) 865 1022 36.00 0.000000011 1 0.310009 0.13

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Oocyst. Fritz HM
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 merozoite. Hehl AB
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Gregory ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_128362)

Species Accession Gene Product
Arabidopsis thaliana AT1G51040   phosphatidylinositol 4-kinase alpha 2
Arabidopsis thaliana AT1G49340   phosphatidylinositol 4-kinase alpha
Brugia malayi Bm1_45855   hypothetical protein
Brugia malayi Bm1_45860   phosphatidylinositol 4-kinase, catalytic, alpha polypeptide
Candida albicans CaO19.1814   Phosphatidylinositol-4-kinase.
Candida albicans CaO19.9377   Phosphatidylinositol-4-kinase.
Caenorhabditis elegans CELE_Y75B8A.24   Protein Y75B8A.24
Dictyostelium discoideum DDB_G0277537   phosphatidylinositol 4-kinase
Drosophila melanogaster Dmel_CG10260   Phosphatidylinositol 4-kinase III alpha
Echinococcus granulosus EgrG_000799800   phosphatidylinositol 4 kinase alpha
Entamoeba histolytica EHI_148700   phosphatidylinositol 4-kinase, putative
Echinococcus multilocularis EmuJ_000799800   phosphatidylinositol 4 kinase alpha
Homo sapiens ENSG00000241973   phosphatidylinositol 4-kinase, catalytic, alpha
Leishmania braziliensis LbrM.29.1540   phosphatidylinositol 4-kinase alpha, putative
Leishmania donovani LdBPK_291550.1   phosphatidylinositol-kinase domain protein, putative
Leishmania infantum LinJ.29.1550   phosphatidylinositol 4-kinase alpha, putative
Leishmania major LmjF.29.1450   phosphatidylinositol 4-kinase alpha, putative
Leishmania mexicana LmxM.08_29.1450   phosphatidylinositol-kinase domain protein, putative
Loa Loa (eye worm) LOAG_15974   hypothetical protein
Loa Loa (eye worm) LOAG_12597   phosphatidylinositol 4-kinase type 3 alpha isoform 1
Loa Loa (eye worm) LOAG_12125   hypothetical protein
Mus musculus ENSMUSG00000041720   phosphatidylinositol 4-kinase, catalytic, alpha polypeptide
Neospora caninum NCLIV_044900   hypothetical protein
Oryza sativa 4333883   Os03g0711200
Saccharomyces cerevisiae YLR305C   1-phosphatidylinositol 4-kinase STT4
Schistosoma japonicum Sjp_0123460   Phosphatidylinositol 4-kinase alpha, putative
Schistosoma japonicum Sjp_0082010   ko:K00888 phosphatidylinositol 4-kinase [EC2.7.1.67], putative
Schistosoma japonicum Sjp_0110720   expressed protein
Schistosoma japonicum Sjp_0131040   Phosphatidylinositol 4-kinase alpha, putative
Schistosoma japonicum Sjp_0129560   Phosphatidylinositol 4-kinase alpha, putative
Schistosoma mansoni Smp_053190   hypothetical protein
Schistosoma mansoni Smp_150660   phosphatidylinositol 4-kinase
Schmidtea mediterranea mk4.002463.00  
Trypanosoma brucei gambiense Tbg972.3.4410   phosphatidylinositol 4-kinase alpha, putative
Trypanosoma brucei Tb927.3.4020   phosphatidylinositol 4-kinase alpha, putative
Trypanosoma congolense TcIL3000_3_2660   phosphatidylinositol 4-kinase alpha, putative
Trypanosoma congolense TcIL3000_0_37620   phosphatidylinositol 4-kinase alpha, putative
Trypanosoma cruzi TcCLB.507531.19   phosphatidylinositol 4-kinase alpha, putative
Trypanosoma cruzi TcCLB.510003.30   phosphatidylinositol 4-kinase alpha, putative
Trypanosoma cruzi TcCLB.507533.10   phosphatidylinositol 4-kinase alpha, putative
Toxoplasma gondii TGME49_228690   phosphatidylinositol 3- and 4-kinase
Trichomonas vaginalis TVAG_161620   phosphatidylinositol 4-kinase, putative
Trichomonas vaginalis TVAG_236630   phosphatidylinositol kinase, putative

Essentiality

TGME49_228690 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.3.4020 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.3.4020 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.3.4020 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.3.4020 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y75B8A.24 Caenorhabditis elegans slow growth wormbase
YLR305C Saccharomyces cerevisiae inviable yeastgenome
TGME49_228690 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens phosphatidylinositol 4-kinase, catalytic, alpha Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0259 0.2531 0.5442
0.0143 0.2865 0.6588
0.0151 0.4477 0.6707
0.0261 0.2525 0.5381
0.0253 0.2519 0.5362
0.00514718 0.411307 0.665081
0.0151 0.4477 0.6707
0.0259 0.2538 0.2449
0.0051 0.2998 0
0.0268 0.2531 0.5464
0.0134 0.407 0.8417
0.0073 0.2587 0.8274
0.026 0.2528 0.5508
0.0093 0.2567 0
0.0146 0.3221 0
0.0047 0.3642 0.4772
0.0268 0.2525 0.5188
0.0261 0.2532 0.5365
0.0264 0.2559 0.5159
0.0261 0.252 0.5189
0.0077 0.395 0
0.0261 0.2532 0.5365
0.0151 0.4477 0.6707

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_228690 (Toxoplasma gondii), phosphatidylinositol 3- and 4-kinase
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