Detailed view for TGME49_228330

Basic information

TDR Targets ID: 260026
Toxoplasma gondii, NLI interacting factor family phosphatase
Source Database / ID:  ToxoDB 

pI: 5.6968 | Length (AA): 1565 | MW (Da): 169721 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03031   NLI interacting factor-like phosphatase

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 12 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
568 751 1ta0 (A) 81 256 21.00 0 0.96 0.35 -0.32
941 1059 2d8m (A) 6 121 24.00 0 0.86 0.29 0.02
207 968 5fsw (A) 43 679 21.00 0 1 -0.0802966 1.9
887 933 5fsw (A) 601 643 49.00 0.13 0.05 0.10954 1.99
1212 1324 5fu5 (A) 1063 1175 5.00 0 0 0.0714463 -0.26
2237 2302 2kr0 (A) 192 259 45.00 0.23 0.04 0.235106 1.69
632 743 5cwq (A) 9 111 34.00 0.086 0.33 0.474265 -1.54
730 875 4xpz (A) 172 318 29.00 0.0039 0.72 0.392391 0.42
771 864 2hhl (A) 60 154 24.00 0 0.23 0.147264 1.04
1311 1387 3pd7 (A) 910 990 22.00 0.087 0.97 0.335301 -0.4
1314 1401 2nte (A) 575 666 24.00 0.74 0.57 0.31933 -0.27
1472 1532 5djn (A) 371 432 34.00 0.3 0.37 0.549678 -1.68

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Bradyzoite. Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Oocyst. Gregory Fritz HM
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 merozoite. Hehl AB
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_129170)

Species Accession Gene Product
Arabidopsis thaliana AT5G58003   C-terminal domain phosphatase-like 4
Brugia malayi Bm1_14230   NLI interacting factor-like phosphatase family protein
Candida albicans CaO19.14034   similar to S. cerevisiae FCP1 (YMR277W) TFIIF interacting Component of CTD Phosphatase
Candida albicans CaO19.6742   similar to S. cerevisiae FCP1 (YMR277W) TFIIF interacting Component of CTD Phosphatase
Caenorhabditis elegans CELE_F36F2.6   Protein FCP-1
Dictyostelium discoideum DDB_G0271690   hypothetical protein
Drosophila melanogaster Dmel_CG12252   CG12252 gene product from transcript CG12252-RB
Echinococcus granulosus EgrG_000495900   RNA polymerase II subunit A C terminal domain
Echinococcus granulosus EgrG_000185200   RNA polymerase II subunit A C terminal domain
Echinococcus multilocularis EmuJ_000372500   hypothetical protein
Echinococcus multilocularis EmuJ_000495900   RNA polymerase II subunit A C terminal domain
Echinococcus multilocularis EmuJ_000185200   RNA polymerase II subunit A C terminal domain
Homo sapiens ENSG00000060069   CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) phosphatase, subunit 1
Loa Loa (eye worm) LOAG_00540   hypothetical protein
Mus musculus ENSMUSG00000033323   CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) phosphatase, subunit 1
Neospora caninum NCLIV_045130   hypothetical protein
Oryza sativa 4338679   Os05g0390500
Saccharomyces cerevisiae YMR277W   Fcp1p
Schistosoma japonicum Sjp_0072470   ko:K01090 protein phosphatase [EC3.1.3.16], putative
Schistosoma mansoni Smp_131560   rna polymerase II ctd phosphatase
Schmidtea mediterranea mk4.003305.02   RNA polymerase II subunit A C-terminal domain phosphatase
Schmidtea mediterranea mk4.001217.03   RNA polymerase II subunit A C-terminal domain phosphatase
Schmidtea mediterranea mk4.021572.00   RNA polymerase II subunit A C-terminal domain phosphatase
Schmidtea mediterranea mk4.004264.00   RNA polymerase II subunit A C-terminal domain phosphatase
Schmidtea mediterranea mk4.016013.00   RNA polymerase II subunit A C-terminal domain phosphatase
Schmidtea mediterranea mk4.021388.00   RNA polymerase II subunit A C-terminal domain phosphatase
Schmidtea mediterranea mk4.002268.00   RNA polymerase II subunit A C-terminal domain phosphatase
Schmidtea mediterranea mk4.022267.02   RNA polymerase II subunit A C-terminal domain phosphatase
Schmidtea mediterranea mk4.010734.00   RNA polymerase II subunit A C-terminal domain phosphatase
Schmidtea mediterranea mk4.020918.00  
Schmidtea mediterranea mk4.000051.03   RNA polymerase II subunit A C-terminal domain phosphatase
Schmidtea mediterranea mk4.003579.04   RNA polymerase II subunit A C-terminal domain phosphatase
Schmidtea mediterranea mk4.003768.04   RNA polymerase II subunit A C-terminal domain phosphatase
Schmidtea mediterranea mk4.004966.00   RNA polymerase II subunit A C-terminal domain phosphatase
Schmidtea mediterranea mk4.000854.10   RNA polymerase II subunit A C-terminal domain phosphatase
Schmidtea mediterranea mk4.009388.00   RNA polymerase II subunit A C-terminal domain phosphatase
Schmidtea mediterranea mk4.022381.00  
Schmidtea mediterranea mk4.012474.00   RNA polymerase II subunit A C-terminal domain phosphatase
Schmidtea mediterranea mk4.000365.06   RNA polymerase II subunit A C-terminal domain phosphatase
Toxoplasma gondii TGME49_228330   NLI interacting factor family phosphatase
Trichomonas vaginalis TVAG_095200   RNA polymerase II ctd phosphatase, putative
Trichomonas vaginalis TVAG_206010   RNA polymerase II ctd phosphatase, putative

Essentiality

TGME49_228330 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
CELE_F36F2.6 Caenorhabditis elegans embryonic lethal wormbase
YMR277W Saccharomyces cerevisiae inviable yeastgenome
TGME49_228330 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_228330 (Toxoplasma gondii), NLI interacting factor family phosphatase
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