Detailed view for PF3D7_1429100

Basic information

TDR Targets ID: 2601
Plasmodium falciparum, apicoplast ribosomal protein L15 precursor, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 10.2919 | Length (AA): 545 | MW (Da): 64759 | Paralog Number: 1

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00828   Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A

Gene Ontology

Mouse over links to read term descriptions.
GO:0000315   organellar large ribosomal subunit  
GO:0020011   apicoplast  
GO:0015934   large ribosomal subunit  
GO:0005840   ribosome  
GO:0005622   intracellular  
GO:0003735   structural constituent of ribosome  
GO:0006412   translation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
315 484 2aw4 (L) 3 143 35.00 0 0.76 0.15 2.71
53 266 4tql (A) 25 221 27.00 0.00016 0.99 0.570661 0.02
70 256 4uos (A) 3 186 24.00 0.0000021 0.97 0.750019 -1.65
319 471 4wfa (I) 6 131 40.00 0 0.35 0.225634 2.05

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Oocyst. Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile early schizont, early trophozoite, late ring, late schizont, late trophozoite, Sporozoite, Female Gametocyte, Male Gametocyte. PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, gametocyte, merozoite, Ring. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs. Otto TD
Show/Hide expression data references
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.

Orthologs

Ortholog group members (OG5_127198)

Species Accession Gene Product
Arabidopsis thaliana AT3G25920   50S ribosomal protein L15
Arabidopsis thaliana AT5G64670   ribosomal protein L18e/L15
Babesia bovis BBOV_IV010420   ribosomal protein L15, putative
Babesia bovis BBOV_III003920   50S ribosomal protein L15, putative
Brugia malayi Bm1_29415   zgc:92856
Candida albicans CaO19.11016   likely mitochondrial ribosomal protein similar to S. cerevisiae MRPL10 (YNL284C) large subunit protein (E. coli L15)
Candida albicans CaO19.3532   likely mitochondrial ribosomal protein similar to S. cerevisiae MRPL10 (YNL284C) large subunit protein (E. coli L15)
Caenorhabditis elegans CELE_Y92H12BR.8   Protein MRPL-15
Chlamydia trachomatis CT_511   50S ribosomal protein L15
Dictyostelium discoideum DDB_G0284405   ribosomal protein L15, mitochondrial
Drosophila melanogaster Dmel_CG5219   mitochondrial ribosomal protein L15
Escherichia coli b3301   50S ribosomal subunit protein L15
Echinococcus granulosus EgrG_000861000   39S ribosomal protein L15 mitochondrial
Echinococcus multilocularis EmuJ_000861000   39S ribosomal protein L15, mitochondrial
Homo sapiens 29088   mitochondrial ribosomal protein L15
Loa Loa (eye worm) LOAG_04259   hypothetical protein
Mycobacterium leprae ML1840c   PROBABLE 50S RIBOSOMAL PROTEIN L15 RPLO
Mus musculus ENSMUSG00000033845   mitochondrial ribosomal protein L15
Mycobacterium tuberculosis Rv0723   50S ribosomal protein L15 RplO
Mycobacterium ulcerans MUL_0813   50S ribosomal protein L15
Neospora caninum NCLIV_012430   ribosomal protein L15, putative
Neospora caninum NCLIV_008150   ribosomal protein L15, putative
Oryza sativa 4332089   Os03g0219900
Oryza sativa 4345238   Os08g0310100
Plasmodium berghei PBANKA_1015600   apicoplast ribosomal protein L15 precursor, putative
Plasmodium berghei PBANKA_1015000   mitochondrial ribosomal protein L15 precursor, putative
Plasmodium falciparum PF3D7_1429100   apicoplast ribosomal protein L15 precursor, putative
Plasmodium falciparum PF3D7_1429700   mitochondrial ribosomal protein L15 precursor, putative
Plasmodium knowlesi PKNH_1329000   ribosomal protein L15, putative
Plasmodium knowlesi PKNH_1329600   ribosomal protein L15, putative
Plasmodium vivax PVX_085090   50S ribosomal protein L15, putative
Plasmodium vivax PVX_085055   ribosomal protein L15, putative
Plasmodium yoelii PY01273   hypothetical protein
Plasmodium yoelii PY00688   Ribosomal protein L15 amino terminal region, putative
Saccharomyces cerevisiae YNL284C   mitochondrial 54S ribosomal protein YmL10/YmL18
Schistosoma japonicum Sjp_0318020   expressed protein
Schistosoma japonicum Sjp_0043960   39S ribosomal protein L15, mitochondrial precursor, putative
Schistosoma mansoni Smp_021080.1   60S ribosomal protein L10
Schmidtea mediterranea mk4.000065.19   39S ribosomal protein L15, mitochondrial
Toxoplasma gondii TGME49_220300   ribosomal protein L15 protein
Toxoplasma gondii TGME49_253800   ribosomal protein L15, putative
Treponema pallidum TP0207   50S ribosomal protein L15
Theileria parva TP01_0686   60S ribosomal protein L15, putative
Theileria parva TP03_0012   hypothetical protein
Wolbachia endosymbiont of Brugia malayi Wbm0323   50S ribosomal protein L15

Essentiality

PF3D7_1429100 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b3301 Escherichia coli essential goodall
CELE_Y92H12BR.8 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y92H12BR.8 Caenorhabditis elegans larval arrest wormbase
CELE_Y92H12BR.8 Caenorhabditis elegans slow growth wormbase
PBANKA_1015000 Plasmodium berghei Essential plasmo
PBANKA_1015600 Plasmodium berghei Essential plasmo
TGME49_253800 Toxoplasma gondii Probably essential sidik
TGME49_220300 Toxoplasma gondii Probably essential sidik
TGME49_253800 Toxoplasma gondii Essentiality uncertain sidik
TGME49_220300 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier PF3D7_1429100 (Plasmodium falciparum), apicoplast ribosomal protein L15 precursor, putative
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