Detailed view for TGME49_233300

Basic information

TDR Targets ID: 260140
Toxoplasma gondii, RhoGAP domain-containing protein
Source Database / ID:  ToxoDB 

pI: 4.5175 | Length (AA): 523 | MW (Da): 56355 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00620   RhoGAP domain
PF13716   Divergent CRAL/TRIO domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  
GO:0007165   signal transduction  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
27 182 2d4q (A) 1560 1714 13.00 0 0.81 0.52 -0.94
28 165 2d4q (A) 1561 1703 12.00 0.00000000063 1 0.51 -0.82
184 387 1xa6 (A) 259 466 17.00 0 1 0.68 -1.77
199 387 1tx4 (A) 49 234 18.00 0 1 0.68 -1.82
32 175 3peg (A) 1565 1716 13.00 0 0.44 0.331335 0.53
176 383 3qis (A) 660 895 19.00 0 1 0.651706 -0.71
194 393 2osa (A) 265 458 21.00 0 1 0.732409 -1.55
455 487 4x8w (H) 1 201 45.00 0 0.05 0.410098 0.78

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 Tachyzoite, ME49 merozoite. Gregory Hehl AB
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_130776)

Species Accession Gene Product
Babesia bovis BBOV_I002300   RhoGAP domain containing protein
Brugia malayi Bm1_09395   RhoGAP domain containing protein
Caenorhabditis elegans CELE_W02B12.8   Protein RGA-1, isoform A
Cryptosporidium hominis Chro.40339   rhoGAP protein
Cryptosporidium parvum cgd4_2980   RhoGAP domain containing protein with a Sec14D domain at the N-terminus
Dictyostelium discoideum DDB_G0281739   RhoGAP domain-containing protein
Drosophila melanogaster Dmel_CG6811   Rho GTPase activating protein at 68F
Echinococcus granulosus EgrG_000657710   rho gtpase activating protein 8
Echinococcus granulosus EgrG_000425300   Rho GTPase activating protein 1
Echinococcus multilocularis EmuJ_000425300   Rho GTPase activating protein 1
Echinococcus multilocularis EmuJ_000657710   rho gtpase activating protein 8
Homo sapiens ENSG00000175220   Rho GTPase activating protein 1
Loa Loa (eye worm) LOAG_06359   RhoGAP domain-containing protein
Mus musculus ENSMUSG00000027247   Rho GTPase activating protein 1
Neospora caninum NCLIV_033090   rhoGAP protein, putative
Plasmodium berghei PBANKA_1205400   rhoGAP GTPase, putative
Plasmodium falciparum PF3D7_1007200   rhoGAP GTPase, putative
Plasmodium knowlesi PKNH_0806000   rhoGAP protein, putative
Plasmodium vivax PVX_094555   Rho-GTPase-activating protein 1, putative
Plasmodium yoelii PY01876   rhoGAP protein
Plasmodium yoelii PY01875   hypothetical protein
Schistosoma japonicum Sjp_0059780   Rho GTPase-activating protein 8, putative
Schistosoma mansoni Smp_006970   hypothetical protein
Schmidtea mediterranea mk4.011355.00   Rho GTPase-activating protein 68F
Schmidtea mediterranea mk4.033029.01  
Toxoplasma gondii TGME49_233300   RhoGAP domain-containing protein
Theileria parva TP03_0096   hypothetical protein
Theileria parva TP03_0097   rhoGAP protein, putative

Essentiality

TGME49_233300 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
PBANKA_1205400 Plasmodium berghei Dispensable plasmo
TGME49_233300 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TGME49_233300 (Toxoplasma gondii), RhoGAP domain-containing protein
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