Detailed view for TGME49_217220

Basic information

TDR Targets ID: 260148
Toxoplasma gondii, zinc finger, C3HC4 type (RING finger) domain-containing protein
Source Database / ID:  ToxoDB 

pI: 5.6706 | Length (AA): 1027 | MW (Da): 115472 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF13920   Zinc finger, C3HC4 type (RING finger)

Gene Ontology

Mouse over links to read term descriptions.
GO:0010390   histone monoubiquitination  
GO:0008270   zinc ion binding  
GO:0005515   protein binding  
GO:0004842   ubiquitin-protein ligase activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 75 5hmo (A) 812 889 17.00 0.0063 0.02 0.189728 -0.39
425 574 4uos (A) 14 165 23.00 0.16 0.46 0.417756 -1.19
480 965 4l6y (B) 6 444 17.00 0 1 0.381123 1.29
958 1025 5trb (A) 907 974 40.00 0.0000006 0.98 0.628912 -0.78
965 1011 2ecj (A) 10 58 38.00 0.56 0.64 0.376464 0.6
969 1012 1rmd (A) 22 65 30.00 0.59 0.74 0.462543 -0.59
973 1020 3lrq (A) 15 63 29.00 0.29 1 0.496438 -0.93

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 Bradyzoite. Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 merozoite, ME49 Oocyst. Gregory Hehl AB Fritz HM
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_128069)

Species Accession Gene Product
Arabidopsis thaliana AT1G55250   E3 ubiquitin-protein ligase HUB2
Arabidopsis thaliana AT2G44950   E3 ubiquitin-protein ligase BRE1-like 1
Babesia bovis BBOV_III010000   hypothetical protein
Brugia malayi Bm1_13375   RE34950p
Brugia malayi Bm1_54905   RING finger protein 20
Candida albicans CaO19.976   Putative coiled-coil protein
Candida albicans CaO19.8591   Putative coiled-coil protein
Caenorhabditis elegans CELE_R05D3.4   Protein RFP-1, isoform B
Cryptosporidium hominis Chro.20099   hypothetical protein
Cryptosporidium parvum cgd2_880   hypothetical protein
Dictyostelium discoideum DDB_G0274241   RING zinc finger-containing protein
Drosophila melanogaster Dmel_CG10542   CG10542 gene product from transcript CG10542-RB
Echinococcus granulosus EgrG_001044600   ribosomal protein s6 kinase beta 1
Echinococcus granulosus EgrG_001178400   Ribosomal protein S6 kinase beta 2
Echinococcus granulosus EgrG_001044900   E3 ubiquitin protein ligase Bre1
Echinococcus multilocularis EmuJ_001044600   ribosomal protein s6 kinase beta 1
Echinococcus multilocularis EmuJ_001178400   Ribosomal protein S6 kinase beta 2
Echinococcus multilocularis EmuJ_001044900   E3 ubiquitin protein ligase Bre1
Homo sapiens ENSG00000155827   ring finger protein 20, E3 ubiquitin protein ligase
Homo sapiens ENSG00000103549   ring finger protein 40, E3 ubiquitin protein ligase
Loa Loa (eye worm) LOAG_02911   RING finger protein 20
Mus musculus ENSMUSG00000028309   ring finger protein 20
Mus musculus ENSMUSG00000030816   ring finger protein 40
Neospora caninum NCLIV_059210   hypothetical protein
Oryza sativa 4349420   Os10g0565600
Plasmodium berghei PBANKA_0102200   trophozoite exported protein 1, putative
Plasmodium falciparum PF3D7_0603400   trophozoite exported protein 1
Plasmodium knowlesi PKNH_1147300   trophozoite exported protein 1, putative
Plasmodium vivax PVX_113335   trophozoite exported protein 1, putative
Plasmodium yoelii PY02581   hypothetical protein
Saccharomyces cerevisiae YDL074C   E3 ubiquitin-protein ligase BRE1
Schistosoma japonicum Sjp_0133950   E3 ubiquitin-protein ligase BRE1B, putative
Schistosoma japonicum Sjp_0048140   ko:K04688 p70 ribosomal S6 kinase, putative
Schistosoma mansoni Smp_136750   serine/threonine protein kinase
Schmidtea mediterranea mk4.010169.02   E3 ubiquitin-protein ligase bre-1
Schmidtea mediterranea mk4.000297.11   E3 ubiquitin-protein ligase bre-1
Schmidtea mediterranea mk4.005144.00   Serine/threonine kinase
Toxoplasma gondii TGME49_217220   zinc finger, C3HC4 type (RING finger) domain-containing protein
Theileria parva TP02_0149   hypothetical protein

Essentiality

TGME49_217220 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
CELE_R05D3.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_R05D3.4 Caenorhabditis elegans larval arrest wormbase
CELE_R05D3.4 Caenorhabditis elegans larval lethal wormbase
CELE_R05D3.4 Caenorhabditis elegans slow growth wormbase
CELE_R05D3.4 Caenorhabditis elegans sterile wormbase
PBANKA_0102200 Plasmodium berghei Dispensable plasmo
TGME49_217220 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_217220 (Toxoplasma gondii), zinc finger, C3HC4 type (RING finger) domain-containing protein
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