Detailed view for TGME49_254010

Basic information

TDR Targets ID: 260246
Toxoplasma gondii, serine carboxypeptidase s28 protein
Source Database / ID:  ToxoDB 

pI: 6.0249 | Length (AA): 738 | MW (Da): 81878 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF05577   Serine carboxypeptidase S28

Gene Ontology

Mouse over links to read term descriptions.
GO:0008236   serine-type peptidase activity  
GO:0006508   proteolysis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
83 209 5d2s (A) 100 184 20.00 0 0.05 -0.0668133 -0.59
204 706 4ebb (A) 28 474 27.00 0 1 0.827272 0.38
206 710 3n2z (B) 47 490 24.00 0 1 0.783982 0.24
208 709 4ebb (A) 32 477 30.00 0 0.98 0.767017 0.75

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 Oocyst, ME49 Bradyzoite. Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite. Gregory Hehl AB
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_127207)

Species Accession Gene Product
Arabidopsis thaliana AT2G18080   probable serine protease EDA2
Arabidopsis thaliana AT4G36195   Serine carboxypeptidase S28 family protein
Arabidopsis thaliana AT4G36190   Serine carboxypeptidase S28 family protein
Brugia malayi Bm1_07005   Serine protease Z688.6 precursor
Caenorhabditis elegans CELE_K12H4.7   Protein K12H4.7, isoform B
Caenorhabditis elegans CELE_Y116F11B.3   Protein PCP-4
Caenorhabditis elegans CELE_F19C7.2   Protein F19C7.2
Caenorhabditis elegans CELE_F23B2.12   Protein PCP-2
Caenorhabditis elegans CELE_F23B2.11   Protein PCP-3
Caenorhabditis elegans CELE_F19C7.4   Protein F19C7.4
Dictyostelium discoideum DDB_G0278299   hypothetical protein
Dictyostelium discoideum DDB_G0290139   peptidase S28 family protein
Dictyostelium discoideum DDB_G0293014   hypothetical protein
Dictyostelium discoideum DDB_G0274663   hypothetical protein
Dictyostelium discoideum DDB_G0290409   hypothetical protein
Dictyostelium discoideum DDB_G0292476   hypothetical protein
Drosophila melanogaster Dmel_CG3734   CG3734 gene product from transcript CG3734-RA
Drosophila melanogaster Dmel_CG9953   CG9953 gene product from transcript CG9953-RA
Drosophila melanogaster Dmel_CG3739   CG3739 gene product from transcript CG3739-RB
Drosophila melanogaster Dmel_CG18493   CG18493 gene product from transcript CG18493-RA
Entamoeba histolytica EHI_037190   serine carboxypeptidase (S28) family protein
Entamoeba histolytica EHI_054530   serine carboxypeptidase (S28) family protein
Entamoeba histolytica EHI_068090   serine carboxypeptidase (S28) family protein
Giardia lamblia GL50803_15871   Serine peptidase, putative
Giardia lamblia GL50803_10843   Thymus-specific serine protease precursor
Homo sapiens ENSG00000112812   protease, serine, 16 (thymus)
Loa Loa (eye worm) LOAG_00786   hypothetical protein
Mus musculus ENSMUSG00000006179   protease, serine 16 (thymus)
Neospora caninum NCLIV_008320   Protein F23B2.12, partially confirmed by transcript evidence, related
Oryza sativa 4349093   Os10g0511600
Oryza sativa 4349092   Os10g0511400
Onchocerca volvulus OVOC319  
Trypanosoma cruzi TcCLB.506425.10   serine carboxypeptidase S28, putative
Trypanosoma cruzi TcCLB.511705.20   putative prolyl carboxypeptidase, putative
Trypanosoma cruzi TcCLB.507681.200   serine carboxypeptidase S28, putative
Toxoplasma gondii TGME49_254010   serine carboxypeptidase s28 protein
Trichomonas vaginalis TVAG_009830   Clan SC, family S28, unassigned serine peptidase
Trichomonas vaginalis TVAG_023320   Clan SC, family S28, unassigned serine peptidase
Trichomonas vaginalis TVAG_462680   Clan SC, family S28, unassigned serine peptidase
Trichomonas vaginalis TVAG_160030   prolylcarboxypeptidase, putative
Trichomonas vaginalis TVAG_229170   prolylcarboxypeptidase, putative
Trichomonas vaginalis TVAG_121720   Clan SC, family S28, unassigned serine peptidase
Trichomonas vaginalis TVAG_399070   Clan SC, family S28, unassigned serine peptidase
Trichomonas vaginalis TVAG_432040   Clan SC, family S28, unassigned serine peptidase
Trichomonas vaginalis TVAG_482780   Clan SC, family S28, unassigned serine peptidase
Trichomonas vaginalis TVAG_229180   conserved hypothetical protein
Trichomonas vaginalis TVAG_263290   Clan SC, family S28, unassigned serine peptidase
Trichomonas vaginalis TVAG_474950   Clan SC, family S28, unassigned serine peptidase
Trichomonas vaginalis TVAG_114870   Clan SC, family S28, unassigned serine peptidase
Trichomonas vaginalis TVAG_160020   Clan SC, family S28, unassigned serine peptidase

Essentiality

TGME49_254010 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
TGME49_254010 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.3

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Dipeptidyl peptidase II 500 aa 24.5% 470 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_254010 (Toxoplasma gondii), serine carboxypeptidase s28 protein
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