Detailed view for TGME49_280610

Basic information

TDR Targets ID: 260289
Toxoplasma gondii, signal recognition particle receptor alpha subunit
Source Database / ID:  ToxoDB 

pI: 7.4379 | Length (AA): 556 | MW (Da): 60815 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00448   SRP54-type protein, GTPase domain
PF04086   Signal recognition particle, alpha subunit, N-terminal

Gene Ontology

Mouse over links to read term descriptions.
GO:0005785   signal recognition particle receptor complex  
GO:0017111   nucleoside-triphosphatase activity  
GO:0005525   GTP binding  
GO:0005515   protein binding  
GO:0005047   signal recognition particle binding  
GO:0003924   GTPase activity  
GO:0000166   nucleotide binding  
GO:0006886   intracellular protein transport  
GO:0006614   SRP-dependent cotranslational protein targeting to membrane  

Metabolic Pathways

Protein export (KEGG)

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
242 564 1zu4 (A) 97 401 26.00 0 1 0.75 -1.29
255 563 1vma (A) 12 292 30.00 0 1 0.95 -1.67
1 129 2fh5 (A) 1 129 32.00 0 1 0.496714 -0.12
1 114 5ck3 (E) 2 127 30.00 0 0.99 0.414736 -0.18
2 90 5ck3 (E) 3 103 36.00 0.27 0.57 0.218572 0.83
2 121 5ck3 (C) 3 134 30.00 0.0000000032 1 0.448727 -0.33
78 238 4uos (A) 9 173 15.00 0.2 0.09 0.440468 -0.72
219 555 3dmd (B) 7 317 38.00 0 1 1.03582 -0.51
262 555 5l3q (B) 333 636 58.00 0 1 1.30328 -1.5

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Hehl AB Fritz HM Sibley/Greg
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_126932)

Species Accession Gene Product
Arabidopsis thaliana AT4G30600   signal recognition particle receptor subunit alpha
Arabidopsis thaliana AT2G45770   cell division protein FtsY
Babesia bovis BBOV_III005280   SRP54-type protein, GTPase domain containing protein
Brugia malayi Bm1_18890   signal recognition particle receptor alpha subunit, putative
Candida albicans CaO19.11434   signal recognition particle receptor
Candida albicans CaO19.3952   signal recognition particle receptor
Caenorhabditis elegans CELE_F38A1.8   Protein F38A1.8
Cryptosporidium hominis Chro.80600   signal recognition particle receptor alpha subunit
Cryptosporidium parvum cgd8_5260   Srp101p GTpase. signal recognition particle receptor alpha subunit
Chlamydia trachomatis CT_820   signal recognition particle receptor FtsY
Dictyostelium discoideum DDB_G0277377   signal recognition particle receptor alpha subunit
Drosophila melanogaster Dmel_CG2522   GTP-binding protein
Escherichia coli b3464   Signal Recognition Particle (SRP) receptor
Echinococcus granulosus EgrG_000774200   signal recognition particle receptor subunit
Entamoeba histolytica EHI_200840   Signal recognition particle receptor alpha subunit, putative
Echinococcus multilocularis EmuJ_000774200   signal recognition particle receptor subunit
Giardia lamblia GL50803_14856   Signal recognition particle receptor
Homo sapiens ENSG00000182934   signal recognition particle receptor (docking protein)
Leishmania braziliensis LbrM.35.3800   signal recognition particle receptor like protein, putative
Leishmania donovani LdBPK_363730.1   signal recognition particle receptor like protein, putative
Leishmania infantum LinJ.36.3730   signal recognition particle receptor like protein, putative
Leishmania major LmjF.36.3570   signal recognition particle receptor like protein, putative
Leishmania mexicana LmxM.36.3570   signal recognition particle receptor like protein, putative
Loa Loa (eye worm) LOAG_03045   srpr protein
Mycobacterium leprae ML1628c   PROBABLE CELL DIVISION PROTEIN FTSY (SRP RECEPTOR) (SIGNAL RECOGNITION PARTICLE RECEPTOR)
Mus musculus ENSMUSG00000032042   signal recognition particle receptor ('docking protein')
Mycobacterium tuberculosis Rv2921c   Probable cell division protein FtsY (SRP receptor) (signal recognition particle receptor)
Mycobacterium ulcerans MUL_2034   cell division protein FtsY
Neospora caninum NCLIV_019540   signal recognition particle protein, putative
Oryza sativa 4345847   Os08g0480100
Oryza sativa 4325387   Os01g0958100
Onchocerca volvulus OVOC1698   Signal recognition particle receptor subunit alpha homolog
Plasmodium berghei PBANKA_1142500   signal recognition particle receptor subunit alpha, putative
Plasmodium falciparum PF3D7_1366600   signal recognition particle receptor subunit alpha, putative
Plasmodium knowlesi PKNH_1104700   signal recognition particle receptor subunit alpha, putative
Plasmodium vivax PVX_115290   signal recognition particle receptor, putative
Plasmodium yoelii PY04912   similar to RIKEN cDNA 1300011P19 gene
Saccharomyces cerevisiae YDR292C   Signal recognition particle receptor subunit alpha
Schistosoma japonicum Sjp_0300740   ko:K03110 signal recognition particle receptor, putative
Schistosoma mansoni Smp_095910.2   signal recognition particle receptor alpha subunit (sr-alpha)
Schistosoma mansoni Smp_095910.1   signal recognition particle receptor alpha subunit (sr-alpha)
Schmidtea mediterranea mk4.001937.02  
Schmidtea mediterranea mk4.002064.00   Signal recognition particle receptor subunit alpha homolog
Trypanosoma brucei gambiense Tbg972.11.11120   signal recognition particle receptor alpha subunit, putative
Trypanosoma brucei Tb927.11.9890   signal recognition particle receptor alpha subunit, putative
Trypanosoma congolense TcIL3000.11.10370   signal recognition particle receptor alpha subunit, putative
Trypanosoma cruzi TcCLB.506337.210   signal recognition particle receptor like protein, putative
Toxoplasma gondii TGME49_280610   signal recognition particle receptor alpha subunit
Treponema pallidum TP0578   cell division protein (ftsY)
Theileria parva TP02_0423   signal recognition particle receptor alpha subunit, putative
Trichomonas vaginalis TVAG_312450   signal recognition particle 54 kD protein, putative
Wolbachia endosymbiont of Brugia malayi Wbm0755   Signal recognition particle GTPase

Essentiality

TGME49_280610 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.1650 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.01.1650 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.1650 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.01.1650 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b3464 Escherichia coli essential goodall
CELE_F38A1.8 Caenorhabditis elegans embryonic lethal wormbase
CELE_F38A1.8 Caenorhabditis elegans larval arrest wormbase
CELE_F38A1.8 Caenorhabditis elegans larval lethal wormbase
CELE_F38A1.8 Caenorhabditis elegans slow growth wormbase
CELE_F38A1.8 Caenorhabditis elegans sterile wormbase
YDR292C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1142500 Plasmodium berghei Essential plasmo
TGME49_280610 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_280610 (Toxoplasma gondii), signal recognition particle receptor alpha subunit
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