Detailed view for TGME49_285770

Basic information

TDR Targets ID: 260299
Toxoplasma gondii, hypothetical protein
Source Database / ID:  ToxoDB 

pI: 10.7275 | Length (AA): 761 | MW (Da): 86210 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF14769   Flagellar C1a complex subunit C1a-32

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
178 242 2kgl (A) 139 193 44.00 0.29 0.05 0.469514 -1.26
192 271 3ted (A) 1042 1116 35.00 0.097 0.07 0.617225 -2.22
205 285 5d80 (K) 26 106 16.00 0 0 0.556739 -3.57

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 Tachyzoite, ME49 Oocyst. Gregory Fritz HM
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile VEG Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_132970)

Species Accession Gene Product
Homo sapiens ENSG00000196118   chromosome 16 open reading frame 93
Leishmania braziliensis LbrM.35.1170   hypothetical protein, conserved
Leishmania donovani LdBPK_361100.1   Flagellar C1a complex subunit C1a-32, putative
Leishmania infantum LinJ.36.1100   hypothetical protein, conserved
Leishmania major LmjF.36.1040   hypothetical protein, conserved
Leishmania mexicana LmxM.36.1040   hypothetical protein, conserved
Mus musculus ENSMUSG00000057176   predicted gene 166
Neospora caninum NCLIV_014610   hypothetical protein, conserved
Schistosoma japonicum Sjp_0204660   expressed protein
Schistosoma mansoni Smp_150410   hypothetical protein
Trypanosoma brucei gambiense Tbg972.10.6620   hypothetical protein, conserved
Trypanosoma brucei Tb927.10.5430   Flagellar C1a complex subunit C1a-32, putative
Trypanosoma congolense TcIL3000_10_4580   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.504097.20   Flagellar C1a complex subunit C1a-32, putative
Trypanosoma cruzi TcCLB.506679.200   Flagellar C1a complex subunit C1a-32, putative
Toxoplasma gondii TGME49_285770   hypothetical protein
Trichomonas vaginalis TVAG_240140   conserved hypothetical protein
Trichomonas vaginalis TVAG_093910   conserved hypothetical protein

Essentiality

TGME49_285770 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.5430 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.5430 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.5430 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.5430 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_285770 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_285770 (Toxoplasma gondii), hypothetical protein
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