Detailed view for TGME49_224040

Basic information

TDR Targets ID: 260364
Toxoplasma gondii, flagellar associated protein
Source Database / ID:  ToxoDB 

pI: 10.145 | Length (AA): 306 | MW (Da): 35706 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00612   IQ calmodulin-binding motif

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
50 137 1x8y (A) 316 385 39.00 0.34 0.09 0.352282 0.49
129 291 5cwm (A) 4 178 18.00 0.55 0.41 0.71298 -0.7
133 302 4uos (A) 2 176 11.00 0.14 0.09 0.723856 -1.4

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
NA% percentile ME49 Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 merozoite. Hehl AB
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile VEG Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_130779)

Species Accession Gene Product
Drosophila melanogaster Dmel_CG13855   CG13855 gene product from transcript CG13855-RA
Echinococcus granulosus EgrG_000812000   IQ domain containing protein
Echinococcus multilocularis EmuJ_000812000   IQ domain containing protein
Giardia lamblia GL50803_10879   Hypothetical protein
Homo sapiens ENSG00000164675   IQ motif and ubiquitin domain containing
Leishmania braziliensis LbrM.22.0790   hypothetical protein, conserved
Leishmania donovani LdBPK_220730.1   hypothetical protein, conserved
Leishmania infantum LinJ.22.0730   hypothetical protein, conserved
Leishmania major LmjF.22.0900   hypothetical protein, conserved
Leishmania mexicana LmxM.22.0900   hypothetical protein, conserved
Mus musculus ENSMUSG00000046192   IQ motif and ubiquitin domain containing
Neospora caninum NCLIV_048480   IQ calmodulin-binding motif domain-containing protein, putative
Schistosoma japonicum Sjp_0062540   IQ and ubiquitin-like domain-containing protein, putative
Schistosoma mansoni Smp_145330   hypothetical protein
Schmidtea mediterranea mk4.011229.00  
Trypanosoma brucei gambiense Tbg972.7.3190   hypothetical protein, conserved
Trypanosoma brucei Tb927.7.2790   Component of motile flagella 10
Trypanosoma congolense TcIL3000_7_2110   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.510525.50   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.511817.120   hypothetical protein, conserved
Toxoplasma gondii TGME49_224040   flagellar associated protein
Trichomonas vaginalis TVAG_110880   conserved hypothetical protein

Essentiality

TGME49_224040 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.2790 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.2790 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.2790 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.2790 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_224040 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_224040 (Toxoplasma gondii), flagellar associated protein
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