Detailed view for TGME49_298630

Basic information

TDR Targets ID: 260368
Toxoplasma gondii, vacuolar transporter chaperone VTC2
Source Database / ID:  ToxoDB 

pI: 8.0436 | Length (AA): 1308 | MW (Da): 141628 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 2

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02656   Domain of unknown function (DUF202)
PF03105   SPX domain
PF09359   VTC domain

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 175 5ijj (A) 1 155 17.00 0.018 0.05 0.191292 -0.17
130 289 5ijh (A) 9 182 21.00 0 0.09 0.340624 -0.81
164 311 5ijj (B) 31 174 21.00 0 0.26 0.20145 -0.04
249 286 5ijh (A) 142 180 47.00 0.037 0.14 0.462952 0.23
399 827 3g3r (A) 199 478 26.00 0 1 -0.0517183 1.28
675 830 3g3o (A) 396 549 28.00 0.51 0.05 -0.0218339 1.59
1167 1305 3dmg (A) 37 171 22.00 0 0.66 0.223569 0.55
1247 1301 4x8w (H) 1 303 55.00 0 0.05 0.0523489 2.73

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 merozoite. Hehl AB
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_130969)

Species Accession Gene Product
Candida albicans CaO19.5455   similar to S. cerevisiae vacuolar transporter chaperone VTC1 and to S. pombe negative regulator of cdc42 GTPase NRF1
Candida albicans CaO19.12910   similar to S. cerevisiae vacuolar transporter chaperone VTC1 and to S. pombe negative regulator of cdc42 GTPase NRF1
Cryptosporidium hominis Chro.60386   G-protein associated signal transduction protein
Cryptosporidium parvum cgd6_3330   membrane associated protein with a SPX domain (SYG1, Pho81 and XPR1) domain at the N-terminus and a vtc1p domain at the C-termin
Dictyostelium discoideum DDB_G0277625   hypothetical protein
Dictyostelium discoideum DDB_G0287759   hypothetical protein
Leishmania braziliensis LbrM.14.1020   hypothetical protein, conserved
Leishmania donovani LdBPK_141100.1   Domain of unknown function (DUF202), putative
Leishmania infantum LinJ.14.1100   hypothetical protein, conserved
Leishmania major LmjF.14.1040   hypothetical protein, conserved
Leishmania mexicana LmxM.14.1040   hypothetical protein, conserved
Plasmodium berghei PBANKA_1444900   vacuolar transporter chaperone, putative
Plasmodium knowlesi PKNH_1449600   vacuolar transporter chaperone, putative
Plasmodium vivax PVX_124150   hypothetical protein, conserved
Plasmodium yoelii PY03770   possible negative regulator of cdc42p-related
Saccharomyces cerevisiae YER072W   Vtc1p
Trypanosoma brucei gambiense Tbg972.7.4360   vacuolar transporter chaperone, putative
Trypanosoma brucei Tb927.7.3900   vacuolar transporter chaperone, putative
Trypanosoma congolense TcIL3000_0_36420   vacuolar transporter chaperone, putative
Trypanosoma cruzi TcCLB.511249.44   vacuolar transporter chaperone, putative
Toxoplasma gondii TGME49_298630   vacuolar transporter chaperone VTC2

Essentiality

TGME49_298630 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.3900 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.3900 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.3900 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.7.3900 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1444900 Plasmodium berghei Dispensable plasmo
TGME49_298630 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_298630 (Toxoplasma gondii), vacuolar transporter chaperone VTC2
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