Detailed view for TGME49_290840

Basic information

TDR Targets ID: 260519
Toxoplasma gondii, serine protease
Source Database / ID:  ToxoDB 

pI: 8.6724 | Length (AA): 960 | MW (Da): 102740 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF13365   Trypsin-like peptidase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  
GO:0004252   serine-type endopeptidase activity  
GO:0003824   catalytic activity  
GO:0006508   proteolysis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
395 781 1ky9 (B) 13 446 20.00 0 1 0.365225 1.15
405 740 3mh4 (B) 11 417 29.00 0.00000000039 1 0.4554 0.84
408 652 4rqz (A) 43 328 23.00 0 1 0.424308 -0.02
409 607 3nwu (B) 162 364 37.00 0 1 0.566692 -0.5
413 957 4fln (A) 113 568 45.00 0 1 0.845108 0.32
432 611 1l1j (A) 68 250 27.00 0 1 0.5276 -0.31
437 610 1y8t (B) 21 220 30.00 0.00000039 1 0.52265 -0.62
872 920 3wkm (A) 231 278 8.00 0.11 0.01 0.107342 -0.27

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 merozoite. Hehl AB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 Oocyst, ME49 Bradyzoite. Fritz HM Sibley/Greg
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_133046)

Species Accession Gene Product
Arabidopsis thaliana AT5G36950   protease Do-like 10
Arabidopsis thaliana AT1G65630   putativeDegP protease 3
Arabidopsis thaliana AT5G40200   DegP protease 9
Babesia bovis BBOV_IV004330   DegP protease, putative
Dictyostelium discoideum DDB_G0281081   hypothetical protein
Neospora caninum NCLIV_042710   Peptidase S1, chymotrypsin:PDZ/DHR/GLGF domain (Precursor), related
Neospora caninum NCLIV_068150   hypothetical protein
Oryza sativa 4330694   Os02g0742500
Oryza sativa 4338806   Os05g0417100
Oryza sativa 4340589   Os06g0234100
Plasmodium falciparum PF3D7_0807700   serine protease DegP
Plasmodium vivax PVX_088155   serine protease DegP, putative
Toxoplasma gondii TGME49_277850   trypsin domain-containing protein
Toxoplasma gondii TGME49_290840   serine protease
Theileria parva TP01_0318   serine protease, putative

Essentiality

TGME49_290840 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
TGME49_277850 Toxoplasma gondii Essentiality uncertain sidik
TGME49_290840 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Anionic trypsin-1 246 aa 20.4% 211 aa Compounds References
Obtained from network model

Ranking Plot

No enough druggable targets predicted through repurposing network model to make a plot

Putative Drugs List

Compound Raw Global Species
0.001 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

13 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_290840 (Toxoplasma gondii), serine protease
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