Detailed view for TGME49_239440

Basic information

TDR Targets ID: 260648
Toxoplasma gondii, protein kinase (incomplete catalytic triad)
Source Database / ID:  ToxoDB 

pI: 9.1708 | Length (AA): 1611 | MW (Da): 175760 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 14 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
778 1092 2etr (A) 70 394 17.00 0 1 0.29 0.44
783 943 2b9h (A) 12 160 31.00 0.000000015 1 0.47 -0.73
1211 1435 1gz8 (A) 36 288 19.00 0 0.74 0.23 0.46
1261 1437 1ia8 (A) 98 269 20.00 0.00000000027 0.32 0.27 -0.17
778 1438 4oli (A) 583 1174 17.00 0 1 0.170604 1.26
780 1092 5j5t (A) 12 307 22.00 0 1 0.288589 0.22
871 943 5loh (A) 108 179 39.00 0.000000027 0.93 0.587713 -0.88
905 942 3aln (A) 213 251 53.00 0.0015 0.96 0.627988 -0.17
1210 1434 1blx (A) 62 301 21.00 0 0.73 0.143965 0.65
1242 1475 5awm (A) 102 361 20.00 0 0.72 0.0845514 0.09
1304 1421 5cek (A) 216 326 43.00 0.000049 0.81 0.202646 1.13
1331 1421 1p4f (A) 179 263 33.00 0.064 0.79 0.429887 -0.53
1332 1486 4nif (A) 573 725 28.00 0.026 0.15 0.234614 0.58
1453 1580 2aao (A) 6 126 12.00 0.022 0 0.0657538 -0.5

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 Bradyzoite. Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Oocyst. Gregory Fritz HM
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 merozoite. Hehl AB
Show/Hide expression data references
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_239694)

Species Accession Gene Product
Neospora caninum NCLIV_016080   protein kinase (incomplete catalytic triad), putative
Toxoplasma gondii TGME49_239440   protein kinase (incomplete catalytic triad)

Essentiality

TGME49_239440 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
TGME49_239440 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0069 0.3067 1
0.0059 1 1
0.0018 0.5 0.5
0.0042 0.5 0.5
0.0007 0.5 0.5
0.0063 1 1
0.0033 0.5 0.5
0.0091 1 0.5
0.0081 0.5 0.5
0.0004 0.5 0.5
0.0062 0.6935 0
0.0059 1 1
0.0039 0.9485 0.5
0.0098 0.3242 0.2614
0.0023 0.5 0.5
0.0007 0.5 0.5
0.0092 1 0.5
0.0029 0.5 0.5
0.0011 1 0.5
0.0008 0.5 0.5
0.0026 0.5 0.5
0.0061 0.6883 0.6656
0.0039 0.5 0.5
0.0036 0.5 0.5
0.0067 0.5 0.5
0.0016 0.5 0.5
0.0027 1 0.5
0.0066 0.3101 0
0.0032 0.5 0.5
0.0093 0.8828 0
0.0088 0.4477 1
0.0081 1 0.5
0.0056 1 0.5
0.0064 0.3377 0
0.0063 0.7244 0.7244
0.0022 0.5 0.5
0.0059 1 1
0.0007 0.5 0.5
0.0012 0.5 0.5
0.0016 0.5 0.5
0.0033 1 1
0.0012 0.5 0.5
0.0012 0.5 0.5
0.0003 0.5 0.5
0.0039 0.5 0.5
0.0032 0.5 0.5
0.0037 1 0.5

Assayability

Assay information

  • Assay for Protein Kinase C (2.7.1.37 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

52 literature references were collected for this gene.

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Gene identifier TGME49_239440 (Toxoplasma gondii), protein kinase (incomplete catalytic triad)
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