Detailed view for TGME49_216960

Basic information

TDR Targets ID: 260656
Toxoplasma gondii, elongation factor Tu GTP binding domain-containing protein
Source Database / ID:  ToxoDB 

pI: 8.2009 | Length (AA): 863 | MW (Da): 92104 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00009   Elongation factor Tu GTP binding domain
PF03144   Elongation factor Tu domain 2

Gene Ontology

Mouse over links to read term descriptions.
GO:0005525   GTP binding  
GO:0003924   GTPase activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
11 455 1jny (A) 4 427 22.00 0 1 0.79 0.09
13 192 1d2e (A) 57 216 34.00 0 1 0.46 -0.74
9 733 5izl (A) 3 508 48.00 0 1 0.687493 1.29
323 410 1xe1 (A) 27 107 22.00 0 0.84 0.30887 -0.3
715 808 2pjp (A) 513 603 12.00 0.011 0.01 0.265522 -0.89

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 Oocyst. Fritz HM
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_130322)

Species Accession Gene Product
Brugia malayi Bm1_55780   Elongation factor Tu GTP binding domain containing protein
Caenorhabditis elegans CELE_C47B2.7   Protein SELB-1, isoform B
Dictyostelium discoideum DDB_G0270386   hypothetical protein
Drosophila melanogaster Dmel_CG9841   CG9841 gene product from transcript CG9841-RA
Echinococcus granulosus EgrG_000516300   selenocysteine specific elongation factor
Echinococcus multilocularis EmuJ_000516300   selenocysteine specific elongation factor
Homo sapiens ENSG00000132394   eukaryotic elongation factor, selenocysteine-tRNA-specific
Leishmania braziliensis LbrM.20.2460   selenocysteine-specific elongation factor, putative
Leishmania donovani LdBPK_342700.1   selenocysteine-specific elongation factor, putative
Leishmania infantum LinJ.34.2700   selenocysteine-specific elongation factor, putative
Leishmania major LmjF.34.2840   selenocysteine-specific elongation factor, putative
Leishmania mexicana LmxM.33.2840   selenocysteine-specific elongation factor, putative
Loa Loa (eye worm) LOAG_00130   hypothetical protein
Mus musculus ENSMUSG00000033216   eukaryotic elongation factor, selenocysteine-tRNA-specific
Neospora caninum NCLIV_059360   elongation factor Tu GTP-binding domain- containing protein, putative
Plasmodium berghei PBANKA_0203500   selenocysteine-specific elongation factor selB homologue, putative
Plasmodium falciparum PF3D7_0110100   selenocysteine-specific elongation factor selB homologue, putative
Plasmodium knowlesi PKNH_0203700   selenocysteine-specific elongation factor selB homologue, putative
Plasmodium vivax PVX_081285   selenocysteine-specific elongation factor selB homologue, putative
Plasmodium yoelii PY00420   selenocysteine-specific elongation factor
Schistosoma japonicum Sjp_0082810   ko:K03231 elongation factor EF-1 alpha subunit, putative
Schistosoma mansoni Smp_176630   selenocysteine-specific elongation factor
Schmidtea mediterranea mk4.020493.00   Selenocysteine-specific elongation factor
Schmidtea mediterranea mk4.001056.01   Selenocysteine-specific elongation factor
Schmidtea mediterranea mk4.009144.00   Selenocysteine-specific elongation factor
Trypanosoma brucei gambiense Tbg972.4.1730   selenocysteine-tRNA-specific elongation factor, putative
Trypanosoma brucei Tb927.4.1820   selenocysteine-specific elongation factor, putative
Trypanosoma congolense TcIL3000_4_1470   selenocysteine-specific elongation factor, putative
Trypanosoma cruzi TcCLB.504013.50   selenocysteine-specific elongation factor, putative
Trypanosoma cruzi TcCLB.510879.70   selenocysteine-specific elongation factor, putative
Toxoplasma gondii TGME49_216960   elongation factor Tu GTP binding domain-containing protein

Essentiality

TGME49_216960 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.1820 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.1820 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.1820 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.4.1820 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_0203500 Plasmodium berghei Dispensable plasmo
TGME49_216960 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_216960 (Toxoplasma gondii), elongation factor Tu GTP binding domain-containing protein
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