Detailed view for TGME49_263580

Basic information

TDR Targets ID: 260657
Toxoplasma gondii, bromodomain-containing protein
Source Database / ID:  ToxoDB 

pI: 5.1501 | Length (AA): 714 | MW (Da): 76521 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00439   Bromodomain
PF12796   Ankyrin repeats (3 copies)
PF13606   Ankyrin repeat

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
125 350 1wdy (A) 27 262 20.00 0 1 0.59 -0.34
159 248 1n0q (A) 3 92 39.00 0.00000000015 1 0.78 -1.73
393 547 2fsa (A) 8 170 22.00 0 0.87 0.5 -0.32
436 546 2d82 (A) 1078 1189 28.00 0 1 0.59 -1
457 546 2fsa (A) 80 169 29.00 0.000000013 1 0.72 -2.1
1 334 4rlv (A) 2484 2801 20.00 0.000000005 1 0.682887 0.21
188 280 2dzn (A) 69 163 28.00 0.2 1 0.572852 -0.94
450 565 3fkm (X) 336 453 49.00 0 1 0.856065 -1.35
569 712 5cwn (A) 26 169 17.00 0 0.02 0.549681 -1.92

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 merozoite. Hehl AB
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_135777)

Species Accession Gene Product
Babesia bovis BBOV_IV010270   bromodomain/ankyrin repeat containing protein
Cryptosporidium hominis Chro.50328   hypothetical protein
Cryptosporidium parvum cgd5_630   protein with 4 ankyrin repeats plus a bromodomain.
Neospora caninum NCLIV_024360   hypothetical protein
Plasmodium berghei PBANKA_0517500   bromodomain protein 1, putative
Plasmodium falciparum PF3D7_1033700   bromodomain protein 1
Plasmodium knowlesi PKNH_0618500   bromodomain protein 1, putative
Plasmodium vivax PVX_111040   hypothetical protein, conserved
Plasmodium yoelii PY03147   hypothetical protein
Plasmodium yoelii PY03146   similar to S. cerevisiae BDF1
Toxoplasma gondii TGME49_263580   bromodomain-containing protein
Theileria parva TP01_0784   hypothetical protein

Essentiality

TGME49_263580 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
PBANKA_0517500 Plasmodium berghei Essential plasmo
TGME49_263580 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_263580 (Toxoplasma gondii), bromodomain-containing protein
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