Detailed view for TGME49_208090

Basic information

TDR Targets ID: 260662
Toxoplasma gondii, 5-formyltetrahydrofolate cyclo-ligase
Source Database / ID:  ToxoDB 

pI: 8.0073 | Length (AA): 473 | MW (Da): 51606 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01812   5-formyltetrahydrofolate cyclo-ligase family

Gene Ontology

Mouse over links to read term descriptions.
GO:0030272   5-formyltetrahydrofolate cyclo-ligase activity  
GO:0005524   ATP binding  
GO:0009396   folic acid and derivative biosynthetic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
267 487 1wkc (A) 3 175 27.00 0 0.99 0.24 0.76
151 254 1udx (A) 65 152 38.00 0.048 0.24 0.345173 0.9
233 459 2jcb (A) 0 183 23.00 0 1 0.489615 0.62
249 459 1wkc (A) 5 167 33.00 0 1 0.414789 0.63
339 383 3hy3 (A) 109 157 49.00 0.0014 0.16 0.386437 1.26

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Hehl AB Fritz HM Sibley/Greg
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_127997)

Species Accession Gene Product
Arabidopsis thaliana AT5G13050   5-formyltetrahydrofolate cycloligase
Candida albicans CaO19.4523   similar to S. cerevisiae YER183C
Candida albicans CaO19.11998   similar to S. cerevisiae YER183C
Caenorhabditis elegans CELE_Y106G6E.4   Protein Y106G6E.4
Chlamydia trachomatis CT_649   formyltetrahydrofolate synthetase
Drosophila melanogaster Dmel_CG34424   CG34424 gene product from transcript CG34424-RA
Escherichia coli b2912   5-formyltetrahydrofolate cyclo-ligase family protein
Echinococcus granulosus EgrG_000773800   5 formyltetrahydrofolate cyclo ligase
Echinococcus multilocularis EmuJ_000773800   5 formyltetrahydrofolate cyclo ligase
Homo sapiens 10588   5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase)
Homo sapiens 100528021   ST20-MTHFS readthrough
Mycobacterium leprae ML0181c   Conserved hypothetical protein
Mus musculus ENSMUSG00000079427   predicted gene 2382
Mus musculus ENSMUSG00000066442   5, 10-methenyltetrahydrofolate synthetase
Mycobacterium tuberculosis Rv0992c   Conserved hypothetical protein
Mycobacterium ulcerans MUL_4694   hypothetical protein
Neospora caninum NCLIV_002980   5-formyltetrahydrofolate cyclo-ligase domain- containing protein, putative
Oryza sativa 4343714   Os07g0578600
Saccharomyces cerevisiae YER183C   5-formyltetrahydrofolate cyclo-ligase
Schistosoma japonicum Sjp_0202250   ko:K01934 5-formyltetrahydrofolate cyclo-ligase [EC6.3.3.2], putative
Schistosoma mansoni Smp_087230   5-formyltetrahydrofolate cyclo-ligase
Schmidtea mediterranea mk4.003655.01   Probable 5-formyltetrahydrofolate cyclo-ligase
Toxoplasma gondii TGME49_208090   5-formyltetrahydrofolate cyclo-ligase
Treponema pallidum TP0694   5,10-methenyltetrahydrofolate synthetase
Wolbachia endosymbiont of Brugia malayi Wbm0256   5-formyltetrahydrofolate cyclo-ligase

Essentiality

TGME49_208090 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu1008 Mycobacterium tuberculosis non-essential nmpdr
b2912 Escherichia coli non-essential goodall
TGME49_208090 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_208090 (Toxoplasma gondii), 5-formyltetrahydrofolate cyclo-ligase
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