Detailed view for TGME49_220860

Basic information

TDR Targets ID: 260780
Toxoplasma gondii, DEAD/DEAH box helicase
Source Database / ID:  ToxoDB 

pI: 6.5046 | Length (AA): 602 | MW (Da): 65158 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00270   DEAD/DEAH box helicase
PF00271   Helicase conserved C-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0008026   ATP-dependent helicase activity  
GO:0005524   ATP binding  
GO:0004386   helicase activity  
GO:0003676   nucleic acid binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
106 601 1gku (B) 1 489 17.00 0 1 0.58 0.34
124 334 1vec (A) 85 276 33.00 0 1 0.85 -1.67
92 589 3ews (A) 59 469 43.00 0 1 1.06104 0.21
120 348 3ber (A) 20 229 29.00 0 1 0.777199 -1.32
123 600 3pey (A) 91 479 43.00 0 1 1.09402 -0.29
126 335 2pl3 (A) 71 264 29.00 0.0000000014 1 0.650837 -0.91
148 503 3fho (A) 16 450 47.00 0 1 0.784362 0.58

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 Bradyzoite. Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst. Gregory Hehl AB Fritz HM
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB
  • Gregory ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_127716)

Species Accession Gene Product
Arabidopsis thaliana AT3G53110   DEAD-box ATP-dependent RNA helicase 38
Babesia bovis BBOV_IV000400   DEAD/DEAH box helicase
Brugia malayi Bm1_20060   DEAD/DEAH box helicase family protein
Candida albicans CaO19.1661   RNA helicase
Candida albicans CaO19.9230   RNA helicase
Caenorhabditis elegans CELE_T07D4.4   Protein DDX-19, isoform D
Cryptosporidium hominis Chro.80546   DEAD-box RNA helicase
Cryptosporidium parvum cgd8_4750   Dbp5p-like eIF4A-1-family RNA SFII helicase
Dictyostelium discoideum DDB_G0281553   DEAD-box RNA helicase
Drosophila melanogaster Dmel_CG17023   Dead box protein 80
Echinococcus granulosus EgrG_000793400   ATP dependent RNA helicase DDX25
Echinococcus multilocularis EmuJ_000793400   ATP dependent RNA helicase DDX25
Homo sapiens ENSG00000157349   DEAD (Asp-Glu-Ala-Asp) box polypeptide 19B
Homo sapiens ENSG00000168872   DEAD (Asp-Glu-Ala-Asp) box polypeptide 19A
Homo sapiens ENSG00000109832   DEAD (Asp-Glu-Ala-Asp) box helicase 25
Loa Loa (eye worm) LOAG_06510   hypothetical protein
Mus musculus ENSMUSG00000033658   DEAD (Asp-Glu-Ala-Asp) box polypeptide 19b
Mus musculus ENSMUSG00000032101   DEAD (Asp-Glu-Ala-Asp) box polypeptide 25
Mus musculus ENSMUSG00000015023   DEAD (Asp-Glu-Ala-Asp) box polypeptide 19a
Neospora caninum NCLIV_004640   DEAD/DEAH box helicase, putative
Oryza sativa 4331680   Os03g0158200
Plasmodium berghei PBANKA_1322700   ATP-dependent RNA helicase DBP5, putative
Plasmodium falciparum PF3D7_1459000   ATP-dependent RNA helicase DBP5
Plasmodium knowlesi PKNH_1222800   ATP-dependent RNA helicase DBP5, putative
Plasmodium vivax PVX_117510   ATP-dependent RNA helicase DBP5, putative
Plasmodium yoelii PY06529   DEAD-box RNA helicase
Saccharomyces cerevisiae YOR046C   ATP-dependent RNA helicase DBP5
Schmidtea mediterranea mk4.010983.00  
Toxoplasma gondii TGME49_220860   DEAD/DEAH box helicase
Theileria parva TP01_0055   DEAD box RNA helicase, putative

Essentiality

TGME49_220860 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
YOR046C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1322700 Plasmodium berghei Essential plasmo
TGME49_220860 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_220860 (Toxoplasma gondii), DEAD/DEAH box helicase
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