Detailed view for TGME49_223620

Basic information

TDR Targets ID: 261019
Toxoplasma gondii, golgi SNARE, putative
Source Database / ID:  ToxoDB 

pI: 9.3143 | Length (AA): 264 | MW (Da): 29139 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF12352   Snare region anchored in the vesicle membrane C-terminus

Gene Ontology

Mouse over links to read term descriptions.
GO:0005484   SNAP receptor activity  
GO:0016020   membrane  
GO:0005794   Golgi apparatus  
GO:0016192   vesicle-mediated transport  
GO:0006886   intracellular protein transport  

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
156 215 1gl2 (C) 139 198 15.00 0.00000057 0 0.4 -0.54
53 186 4fla (A) 189 298 31.00 0.15 0.16 0.505976 1.1

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Oocyst. Fritz HM
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Show/Hide expression data references
  • Gregory ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_128931)

Species Accession Gene Product
Arabidopsis thaliana AT2G36900   membrin 11
Arabidopsis thaliana AT5G50440   membrin 12
Brugia malayi Bm1_32550   membrin
Candida albicans CaO19.10457   similar to synaptobrevin v-SNARE protein, ER_Golgi transport
Candida albicans CaO19.2940   similar to synaptobrevin v-SNARE protein, ER_Golgi transport
Caenorhabditis elegans CELE_B0272.2   Protein MEMB-1
Cryptosporidium hominis Chro.10325   Golgi SNARE protein
Cryptosporidium parvum cgd1_2920   golgi transport SNARE BOS1 secretory pathway protein, possible transmembrane or GPI anchor at C-terminus
Dictyostelium discoideum DDB_G0268452   v-SNARE family protein
Drosophila melanogaster Dmel_CG4780   CG4780 gene product from transcript CG4780-RA
Homo sapiens ENSG00000108433   golgi SNAP receptor complex member 2
Leishmania braziliensis LbrM.19.1080   SNARE protein, putative
Leishmania donovani LdBPK_190770.1   SNARE protein, putative
Leishmania infantum LinJ.19.0770   SNARE protein, putative
Leishmania major LmjF.19.0770   SNARE protein, putative
Leishmania mexicana LmxM.19.0770   SNARE protein, putative
Loa Loa (eye worm) LOAG_04246   hypothetical protein
Mus musculus ENSMUSG00000020946   golgi SNAP receptor complex member 2
Oryza sativa 4333608   Os03g0655200
Onchocerca volvulus OVOC3057   Putative Golgi SNAP receptor complex member 2
Saccharomyces cerevisiae YLR078C   Bos1p
Schmidtea mediterranea mk4.001605.02  
Schmidtea mediterranea mk4.018664.01  
Trypanosoma brucei gambiense Tbg972.10.18940   SNARE protein, putative
Trypanosoma brucei Tb927.10.15500   SNARE protein, putative
Trypanosoma congolense TcIL3000_10_13290   SNARE protein, putative
Trypanosoma cruzi TcCLB.471901.20   SNARE protein, putative
Toxoplasma gondii TGME49_223620   golgi SNARE, putative

Essentiality

TGME49_223620 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.15500 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.15500 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.15500 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.15500 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_B0272.2 Caenorhabditis elegans embryonic lethal wormbase
YLR078C Saccharomyces cerevisiae inviable yeastgenome
TGME49_223620 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TGME49_223620 (Toxoplasma gondii), golgi SNARE, putative
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