Detailed view for TGME49_268910

Basic information

TDR Targets ID: 261138
Toxoplasma gondii, signal peptidase I protein
Source Database / ID:  ToxoDB 

pI: 9.7514 | Length (AA): 215 | MW (Da): 23730 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00717   Peptidase S24-like

Gene Ontology

Mouse over links to read term descriptions.
GO:0016020   membrane  
GO:0016021   integral to membrane  
GO:0008236   serine-type peptidase activity  
GO:0006508   proteolysis  

Metabolic Pathways

Protein export (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
104 208 2e3j (A) 117 234 34.00 0.57 0.11 0.410672 1.59
108 186 4me8 (A) 55 165 37.00 0 0.99 0.482142 -0.81

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Bradyzoite. Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst. Gregory Hehl AB Fritz HM
Show/Hide expression data references
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_128814)

Species Accession Gene Product
Arabidopsis thaliana AT1G23465   peptidase-S24/S26 domain-containing protein
Arabidopsis thaliana AT1G53530   peptidase-S24/S26 domain-containing protein
Arabidopsis thaliana AT1G29960   peptidase-S24/S26 domain-containing protein
Babesia bovis BBOV_I004260   conserved hypothetical protein
Brugia malayi Bm1_15285   Peptidase S24-like domain containing protein
Candida albicans CaO19.3061   Mitochondrial inner membrane protease
Candida albicans CaO19.10579   Mitochondrial inner membrane protease
Caenorhabditis elegans CELE_C24H11.6   Protein IMMP-1
Cryptosporidium hominis Chro.40078   hypothetical protein
Cryptosporidium parvum cgd4_620   hypothetical protein
Dictyostelium discoideum DDB_G0283049   hypothetical protein
Drosophila melanogaster Dmel_CG9240   CG9240 gene product from transcript CG9240-RA
Echinococcus granulosus EgrG_001050600   mitochondrial inner membrane protease subunit
Echinococcus multilocularis EmuJ_001050600   mitochondrial inner membrane protease subunit
Homo sapiens ENSG00000148950   IMP1 inner mitochondrial membrane peptidase-like (S. cerevisiae)
Loa Loa (eye worm) LOAG_07003   inner mitochondrial membrane protease family member
Mus musculus ENSMUSG00000042670   IMP1 inner mitochondrial membrane peptidase-like (S. cerevisiae)
Neospora caninum NCLIV_037440   Mitochondrial inner membrane signal peptidase, related
Oryza sativa 4350939   Os11g0620000
Plasmodium berghei PBANKA_1418700   microsomal signal peptidase, putative
Plasmodium falciparum PF3D7_1320400   type I signal peptidase
Plasmodium knowlesi PKNH_1421000   signal peptidase, putative
Plasmodium vivax PVX_122830   big signal peptidase, putative
Plasmodium yoelii PY07139   hypothetical protein
Saccharomyces cerevisiae YMR150C   Imp1p
Schistosoma japonicum Sjp_0058700   ko:K09647 mitochondrial inner membrane protease subunit 1, putative
Schistosoma mansoni Smp_018430   mitochondrial signal peptidase (S26 family)
Schmidtea mediterranea mk4.004706.02   Mitochondrial inner membrane protease subunit 1
Toxoplasma gondii TGME49_268910   signal peptidase I protein
Theileria parva TP01_0970   mitochondrial membrane protease subunit 2, putative

Essentiality

TGME49_268910 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
TGME49_268910 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Staphylococcus aureus Signal peptidase IB 191 aa 23.9% 176 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_268910 (Toxoplasma gondii), signal peptidase I protein
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