Detailed view for TGME49_269680

Basic information

TDR Targets ID: 261221
Toxoplasma gondii, acyl-CoA carboxyltransferase beta chain, putative
Source Database / ID:  ToxoDB 

pI: 9.5257 | Length (AA): 812 | MW (Da): 87950 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01039   Carboxyl transferase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016874   ligase activity  

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
231 812 3u9r (B) 32 563 59.00 0 1 1.29715 -0.08
241 812 3u9t (B) 41 563 60.00 0 1 0.977534 0.69
246 812 4q0g (A) 13 529 55.00 0 1 1.25038 -0.1
279 502 2f9y (B) 53 284 24.00 0 0.99 0.533262 -0.14

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
NA% percentile VEG Tachyzoite, ME49 Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Oocyst. Fritz HM
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile ME49 merozoite, ME49 Bradyzoite. Hehl AB Sibley/Greg
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_129211)

Species Accession Gene Product
Arabidopsis thaliana AT4G34030   methylcrotonoyl-CoA carboxylase beta chain
Caenorhabditis elegans CELE_F02A9.4   Protein F02A9.4, isoform A
Dictyostelium discoideum DDB_G0271960   3-methylcrotonyl-CoA:carbon dioxide ligase beta subunit
Drosophila melanogaster Dmel_CG3267   CG3267 gene product from transcript CG3267-RA
Homo sapiens ENSG00000131844   methylcrotonoyl-CoA carboxylase 2 (beta)
Leishmania braziliensis LbrM.11.0370   3-methylcrotonoyl-CoA carboxylase beta subunit, putative
Leishmania donovani LdBPK_110600.1   3-methylcrotonoyl-CoA carboxylase beta subunit, putative
Leishmania infantum LinJ.11.0600   3-methylcrotonoyl-CoA carboxylase beta subunit, putative
Leishmania major LmjF.11.0590   3-methylcrotonoyl-CoA carboxylase beta subunit, putative
Leishmania mexicana LmxM.11.0590   3-methylcrotonoyl-CoA carboxylase beta subunit, putative
Mus musculus ENSMUSG00000021646   methylcrotonoyl-Coenzyme A carboxylase 2 (beta)
Mycobacterium tuberculosis Rv2502c   Probable acetyl-/propionyl-CoA carboxylase (beta subunit) AccD1
Neospora caninum NCLIV_036840   acyl-CoA carboxyltransferase beta chain, putative
Oryza sativa 4345605   Os08g0424200
Schmidtea mediterranea mk4.088796.01  
Schmidtea mediterranea mk4.041435.00   Probable methylcrotonoyl-CoA carboxylase beta chain, mitochondrial
Schmidtea mediterranea mk4.002162.05   Probable methylcrotonoyl-CoA carboxylase beta chain, mitochondrial
Trypanosoma brucei gambiense Tbg972.11.7500   3-methylcrotonoyl-CoA carboxylase beta subunit, putative
Trypanosoma brucei Tb927.11.6630   3-methylcrotonoyl-CoA carboxylase beta subunit, putative
Trypanosoma congolense TcIL3000_0_44270   3-methylcrotonoyl-CoA carboxylase beta subunit, putative
Trypanosoma cruzi TcCLB.508799.170   3-methylcrotonoyl-CoA carboxylase beta subunit, putative
Trypanosoma cruzi TcCLB.506773.40   3-methylcrotonoyl-CoA carboxylase beta subunit, putative
Toxoplasma gondii TGME49_269680   acyl-CoA carboxyltransferase beta chain, putative

Essentiality

TGME49_269680 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.02.4480 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.4480 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.02.4480 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.02.4480 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F02A9.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_F02A9.4 Caenorhabditis elegans larval arrest wormbase
CELE_F02A9.4 Caenorhabditis elegans slow growth wormbase
TGME49_269680 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_269680 (Toxoplasma gondii), acyl-CoA carboxyltransferase beta chain, putative
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