Detailed view for TGME49_237500

Basic information

TDR Targets ID: 261297
Toxoplasma gondii, protein phosphatase 2C domain-containing protein
Source Database / ID:  ToxoDB 

pI: 6.3288 | Length (AA): 909 | MW (Da): 97305 | Paralog Number: 1

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00481   Protein phosphatase 2C

Gene Ontology

Mouse over links to read term descriptions.
GO:0043169   cation binding  
GO:0004722   protein serine/threonine phosphatase activity  
GO:0003824   catalytic activity  
GO:0006470   protein amino acid dephosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
569 939 1a6q () 7 345 26.00 0 1 0.49 -0.17
589 884 1a6q () 27 288 35.00 0 1 0.62 -0.34
59 185 1wdi (A) 19 152 34.00 0.52 0.21 0.075014 1.4
540 859 4jnd (A) 111 432 21.00 0 1 0.453135 0.29
574 864 2isn (A) 3 342 45.00 0.043 1 0.748832 -0.65

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 2ISN:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 merozoite. Gregory Hehl AB
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB
  • Gregory ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_136796)

Species Accession Gene Product
Leishmania braziliensis LbrM.30.0420   phosphatase 2C, putative
Leishmania donovani LdBPK_300380.1   phosphatase 2C, putative
Leishmania infantum LinJ.30.0380   phosphatase 2C, putative
Leishmania major LmjF.30.0380   phosphatase 2C, putative
Leishmania mexicana LmxM.29.0380   phosphatase 2C, putative
Neospora caninum NCLIV_051240   protein phosphatase 2C, putative
Neospora caninum NCLIV_068640   protein phosphatase 2C, putative
Plasmodium berghei PBANKA_1318700   protein phosphatase PPM3
Plasmodium falciparum PF3D7_1455000   protein phosphatase PPM3, putative
Plasmodium knowlesi PKNH_1227100   protein phosphatase PPM3, putative
Plasmodium vivax PVX_117730   protein phosphatase PPM3, putative
Plasmodium yoelii PY00861   unnamed protein product
Trypanosoma brucei gambiense Tbg972.6.1500   protein phosphatase 2C, putative
Trypanosoma brucei Tb927.6.1800   protein phosphatase 2C, putative
Trypanosoma congolense TcIL3000_6_1380   protein phosphatase 2C, putative
Trypanosoma cruzi TcCLB.511491.100   phosphatase 2C, putative
Toxoplasma gondii TGME49_276920   protein phosphatase 2C domain-containing protein
Toxoplasma gondii TGME49_237500   protein phosphatase 2C domain-containing protein

Essentiality

TGME49_237500 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.1800 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.6.1800 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.6.1800 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.1800 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1318700 Plasmodium berghei Dispensable plasmo
TGME49_237500 this record Toxoplasma gondii Probably non-essential sidik
TGME49_276920 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.4

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Calcineurin (3.1.3.16 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

6 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier TGME49_237500 (Toxoplasma gondii), protein phosphatase 2C domain-containing protein
Title for this comment
Comment