Detailed view for TGME49_239130

Basic information

TDR Targets ID: 261316
Toxoplasma gondii, Tyrosine kinase-like (TKL) protein
Source Database / ID:  ToxoDB 

pI: 9.6932 | Length (AA): 1673 | MW (Da): 181852 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain
PF07647   SAM domain (Sterile alpha motif)

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0005524   ATP binding  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1072 1133 3bq7 (A) 5 65 26.00 0.16 1 0.453859 -0.87
1109 1663 2ozo (A) 21 605 23.00 0 1 0.362539 1.19
1471 1520 5b2m (A) 242 306 50.00 0.0036 0.73 0.401686 1.05
1540 1656 3f82 (A) 1207 1342 25.00 0 0.93 0.432134 -0.68
1541 1653 1p4o (A) 1126 1238 27.00 0 0.81 0.440743 -0.71

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Oocyst. Fritz HM
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_130251)

Species Accession Gene Product
Arabidopsis thaliana AT1G73660   MAPKKK-like kinase
Arabidopsis thaliana AT5G11850   protein kinase superfamily protein
Arabidopsis thaliana AT1G18160   protein kinase family protein
Arabidopsis thaliana AT1G08720   serine/threonine-protein kinase EDR1
Caenorhabditis elegans CELE_R13F6.6   Protein ZAK-1, isoform B
Cryptosporidium hominis Chro.80284   protein kinase
Cryptosporidium parvum cgd8_2430   Ser/Thr protein kinase with MORN repeats at the N-terminus and a sterile alpha motif (SAM_ domain
Homo sapiens ENSG00000091436   sterile alpha motif and leucine zipper containing kinase AZK
Mus musculus ENSMUSG00000004085   sterile alpha motif and leucine zipper containing kinase AZK
Neospora caninum NCLIV_015900   Protein kinase domain-containing protein (EC 2.7.11.-), related
Oryza sativa 4348694   Os10g0430900
Oryza sativa 4331696   Os03g0160100
Oryza sativa 4328754   Os02g0220700
Oryza sativa 4340578   Os06g0232100
Oryza sativa 4330701   Os02g0743500
Plasmodium berghei PBANKA_0308500   tyrosine kinase-like protein, putative
Plasmodium falciparum PF3D7_0211700   tyrosine kinase-like protein, putative
Plasmodium knowlesi PKNH_0409500   tyrosine kinase-like protein, putative
Plasmodium vivax PVX_002945   tyrosine kinase-like protein, putative
Plasmodium yoelii PY02503   MORN repeat, putative
Toxoplasma gondii TGME49_239130   Tyrosine kinase-like (TKL) protein

Essentiality

TGME49_239130 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
PBANKA_0308500 Plasmodium berghei Dispensable plasmo
TGME49_239130 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens sterile alpha motif and leucine zipper containing kinase AZK Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Jak1 protein 210 aa 24.7% 231 aa Compounds References
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 26.3% 205 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0093 0.8828 0
0.0092 1 0.5
0.0039 0.9485 0.5
0.0007 0.5 0.5
0.0088 0.4477 1
0.0061 0.6883 0.6656
0.0027 1 0.5
0.0081 1 0.5
0.0042 0.5 0.5
0.0098 0.3242 0.2614
0.0032 0.5 0.5
0.0064 0.3377 0
0.0016 0.5 0.5
0.0012 0.5 0.5
0.0023 0.5 0.5
0.0004 0.5 0.5
0.0012 0.5 0.5
0.0032 0.5 0.5
0.0029 0.5 0.5
0.0069 0.3067 1
0.0016 0.5 0.5
0.0008 0.5 0.5
0.0063 0.7244 0.7244
0.0056 1 0.5
0.0033 0.5 0.5
0.0081 0.5 0.5
0.0012 0.5 0.5
0.0036 0.5 0.5
0.0059 1 1
0.0022 0.5 0.5
0.0066 0.3101 0
0.0018 0.5 0.5
0.0039 0.5 0.5
0.0011 1 0.5
0.0091 1 0.5
0.0063 1 1
0.0039 0.5 0.5
0.0026 0.5 0.5
0.0037 1 0.5
0.0062 0.6935 0
0.0067 0.5 0.5
0.0059 1 1
0.0007 0.5 0.5
0.0007 0.5 0.5
0.0003 0.5 0.5
0.0033 1 1
0.0059 1 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_239130 (Toxoplasma gondii), Tyrosine kinase-like (TKL) protein
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