Detailed view for TGME49_285850

Basic information

TDR Targets ID: 261604
Toxoplasma gondii, peptidyl-prolyl cis-trans isomerase, FKBP-type domain-containing protein
Source Database / ID:  ToxoDB 

pI: 8.9996 | Length (AA): 428 | MW (Da): 46099 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00254   FKBP-type peptidyl-prolyl cis-trans isomerase

Gene Ontology

Mouse over links to read term descriptions.
GO:0006457   protein folding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
118 295 4uos (A) 11 185 13.00 0 0.04 0.662288 -1.65
125 315 5cwq (A) 4 221 29.00 0.0041 0.71 0.735462 -0.86
168 260 1oqy (A) 77 160 32.00 0.43 0 0.33749 0.92
304 427 2y78 (A) 0 113 44.00 0 1 0.69112 -0.27
336 427 2pbc (A) 41 136 46.00 0 1 0.741353 -0.68
339 426 3jym (A) 288 382 30.00 0 0.99 0.561007 -0.72
367 425 2f4e (A) 95 156 34.00 0.024 0.3 0.41805 0.32

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 merozoite. Hehl AB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 Bradyzoite. Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile ME49 Oocyst. Fritz HM
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_129720)

Species Accession Gene Product
Arabidopsis thaliana AT4G25340   peptidyl-prolyl cis-trans isomerase FKBP53
Arabidopsis thaliana AT3G12340   FKBP-like peptidyl-prolyl cis-trans isomerase family protein
Candida albicans CaO19.1030   FKBP-type peptidylprolyl cis/trans isomerase similar to S. cerevisiae FPR3 (YML074C) nucleolar proline rotamase
Candida albicans CaO19.8632   FKBP-type peptidylprolyl cis/trans isomerase similar to S. cerevisiae FPR3 (YML074C) nucleolar proline rotamase
Cryptosporidium hominis Chro.70034   immunophilin/FKBP-type peptidyl-prolyl cis-trans isomerase
Cryptosporidium parvum cgd7_210   fkbp
Dictyostelium discoideum DDB_G0285357   FKBP-type peptidylprolyl cis-trans isomerase
Drosophila melanogaster Dmel_CG6226   FK506-binding protein 1
Escherichia coli b3347   FKBP-type peptidyl-prolyl cis-trans isomerase (rotamase)
Giardia lamblia GL50803_10570   FKBP-type peptidyl-prolyl cis-trans isomerase
Neospora caninum NCLIV_014540   hypothetical protein
Oryza sativa 4335968   Os04g0446500
Saccharomyces cerevisiae YLR449W   peptidylprolyl isomerase FPR4
Saccharomyces cerevisiae YML074C   peptidylprolyl isomerase FPR3
Toxoplasma gondii TGME49_285850   peptidyl-prolyl cis-trans isomerase, FKBP-type domain-containing protein

Essentiality

TGME49_285850 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
b3347 Escherichia coli non-essential goodall
TGME49_285850 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus FK506-binding protein 1A 108 aa 38.2% 89 aa Compounds References
Gallus gallus FK506 binding protein 12 108 aa 39.8% 103 aa Compounds References
Legionella pneumophila Outer membrane protein MIP 233 aa 28.3% 187 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for FK-Binding Protein Peptidyl-Prolyl Isomerase Activity (5.2.1.8 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

6 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TGME49_285850 (Toxoplasma gondii), peptidyl-prolyl cis-trans isomerase, FKBP-type domain-containing protein
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