Detailed view for TGME49_269940

Basic information

TDR Targets ID: 261640
Toxoplasma gondii, zinc finger motif, C2HC5-type protein
Source Database / ID:  ToxoDB 

pI: 5.246 | Length (AA): 1206 | MW (Da): 134622 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04266   ASCH domain
PF06221   Putative zinc finger motif, C2HC5-type

Gene Ontology

Mouse over links to read term descriptions.
GO:0006355   regulation of transcription, DNA-dependent  
GO:0005634   nucleus  
GO:0030528   transcription regulator activity  
GO:0008270   zinc ion binding  
GO:0045449   regulation of transcription  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
5 358 4k6j (A) 721 1127 11.00 0 0.04 0.309032 -0.05
39 265 5cwp (A) 5 229 14.00 0.067 0.07 0.330226 -0.77
234 903 4y01 (A) 51 663 10.00 0 0.99 0.508056 0.97
297 363 2maj (A) 317 387 25.00 0 0.01 0.513056 -3.16
298 359 5hhe (D) 176 237 24.00 0 0.01 0.69791 -4.55
298 363 5hmo (A) 814 887 38.00 0.00033 0.03 0.654126 -3.42
1011 1186 2e5o (A) 8 152 33.00 0.000000000018 0.98 0.207937 0.74

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Tachyzoite, ME49 Oocyst. Gregory Fritz HM
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile VEG Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_128655)

Species Accession Gene Product
Arabidopsis thaliana AT3G47610   transcription regulator/ zinc ion binding protein
Babesia bovis BBOV_III002540   conserved hypothetical protein
Brugia malayi Bm1_42910   hypothetical protein
Brugia malayi Bm1_42915   Zinc finger motif, C2HC5-type family protein
Candida albicans CaO19.9927   similar to S. cerevisiae YKR023W
Candida albicans CaO19.2391   similar to S. cerevisiae YKR023W
Caenorhabditis elegans CELE_Y53F4B.15   Protein ASC-1
Cryptosporidium hominis Chro.30398   hypothetical protein
Cryptosporidium parvum cgd3_3530   hypothetical protein
Dictyostelium discoideum DDB_G0269884   hypothetical protein
Drosophila melanogaster Dmel_CG11710   CG11710 gene product from transcript CG11710-RC
Echinococcus granulosus EgrG_000142800   activating signal cointegrator 1
Entamoeba histolytica EHI_104490   hypothetical protein
Echinococcus multilocularis EmuJ_000141800   activating signal cointegrator 1
Echinococcus multilocularis EmuJ_000142800   activating signal cointegrator 1
Giardia lamblia GL50803_27694   Hypothetical protein
Homo sapiens ENSG00000103671   thyroid hormone receptor interactor 4
Leishmania braziliensis LbrM.08.0070   hypothetical protein, conserved
Leishmania donovani LdBPK_080070.1   Putative zinc finger motif, C2HC5-type/ASCH domain containing protein, putative
Leishmania infantum LinJ.08.0070   hypothetical protein, conserved
Leishmania major LmjF.08.0070   hypothetical protein, conserved
Leishmania mexicana LmxM.08.0070  
Mus musculus ENSMUSG00000032386   thyroid hormone receptor interactor 4
Neospora caninum NCLIV_036650   putative zinc finger motif, C2HC5-type domain- containing protein
Oryza sativa 4347045   Os09g0413100
Saccharomyces cerevisiae YKR023W   hypothetical protein
Schistosoma japonicum Sjp_0308590   Activating signal cointegrator 1, putative
Schistosoma japonicum Sjp_0071860   Activating signal cointegrator 1, putative
Schistosoma mansoni Smp_167590   hypothetical protein
Schmidtea mediterranea mk4.001842.00   Activating signal cointegrator 1
Schmidtea mediterranea mk4.001842.01  
Trypanosoma brucei gambiense Tbg972.5.5010   hypothetical protein, conserved
Trypanosoma brucei Tb927.5.3590   Putative zinc finger motif, C2HC5-type/ASCH domain containing protein, putative
Trypanosoma congolense TcIL3000_5_4030   hypothetical protein, conserved
Trypanosoma congolense TcIL3000_0_04470   ASCH domain containing protein, putative
Trypanosoma cruzi TcCLB.507811.40   Putative zinc finger motif, C2HC5-type/ASCH domain containing protein, putative
Trypanosoma cruzi TcCLB.511649.160   Putative zinc finger motif, C2HC5-type/ASCH domain containing protein, putative
Toxoplasma gondii TGME49_269940   zinc finger motif, C2HC5-type protein
Trichomonas vaginalis TVAG_056660   conserved hypothetical protein

Essentiality

TGME49_269940 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.3590 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.3590 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.5.3590 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.3590 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_269940 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier TGME49_269940 (Toxoplasma gondii), zinc finger motif, C2HC5-type protein
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