Detailed view for TGME49_254570

Basic information

TDR Targets ID: 261805
Toxoplasma gondii, hypothetical protein
Source Database / ID:  ToxoDB 

pI: 4.5917 | Length (AA): 1972 | MW (Da): 221185 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 11 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
3 204 1oqy (A) 87 299 28.00 0.47 0.85 0.0813341 1.8
28 113 3q8t (A) 173 258 12.00 0.52 0 0.150711 -0.19
107 324 4f61 (I) 36 237 31.00 0.31 0.03 0.118448 1.49
125 311 5cwg (A) 9 195 28.00 0.092 0.95 0.321728 0.12
149 363 4gnk (E) 956 1189 28.00 0.009 0.27 0.171926 0.68
221 359 2n5e (A) 58 235 38.00 0.8 0.25 0.225387 0.84
1038 1217 4uos (A) 5 185 16.00 0 0.14 0.306378 -2.04
1085 1168 3v6i (B) 26 100 44.00 0.87 0.03 0.349496 -0.2
1158 1517 5lm2 (B) 6 345 14.00 0 1 0.186256 -0.05
1339 1498 2v1y (B) 437 596 15.00 0 1 0.304836 -1.44
1598 1904 5hm5 (A) 386 797 24.00 0.45 0.49 0.26758 0.51

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 merozoite. Hehl AB
Show/Hide expression data references
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_129652)

Species Accession Gene Product
Arabidopsis thaliana AT1G79280   nuclear pore anchor
Brugia malayi Bm1_11925   Viral A-type inclusion protein repeat containing protein
Candida albicans CaO19.4683   myosin-like protein
Candida albicans CaO19.12152   myosin-like protein
Caenorhabditis elegans CELE_R07G3.3   Protein NPP-21, isoform B
Dictyostelium discoideum DDB_G0288073   hypothetical protein
Drosophila melanogaster Dmel_CG8274   Megator
Echinococcus granulosus EgrG_000102000   nucleoprotein TPR
Echinococcus multilocularis EmuJ_000102000   nucleoprotein TPR
Homo sapiens ENSG00000047410   translocated promoter region, nuclear basket protein
Loa Loa (eye worm) LOAG_06949   hypothetical protein
Loa Loa (eye worm) LOAG_08920   viral A-type inclusion protein repeat containing protein
Mus musculus ENSMUSG00000006005   translocated promoter region, nuclear basket protein
Neospora caninum NCLIV_008850   hypothetical protein, conserved
Oryza sativa 4330687   Os02g0741500
Onchocerca volvulus OVOC10101  
Schistosoma japonicum Sjp_0033840   ko:K09291 nucleoprotein TPR, putative
Schistosoma japonicum Sjp_0088580   ko:K09291 nucleoprotein TPR, putative
Schistosoma mansoni Smp_160850   Tpr
Schmidtea mediterranea mk4.002240.06   Nucleoprotein TPR
Schmidtea mediterranea mk4.002240.07   Nucleoprotein TPR
Toxoplasma gondii TGME49_254570   hypothetical protein

Essentiality

TGME49_254570 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
CELE_R07G3.3 Caenorhabditis elegans embryonic lethal wormbase
CELE_R07G3.3 Caenorhabditis elegans larval arrest wormbase
TGME49_254570 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_254570 (Toxoplasma gondii), hypothetical protein
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