Detailed view for TGME49_229690

Basic information

TDR Targets ID: 261844
Toxoplasma gondii, autophagy-related protein 7 atg7, putative

Source Database / ID:  ToxoDB 

pI: 6.0422 | Length (AA): 1332 | MW (Da): 143702 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00899   ThiF family
PF16420   Ubiquitin-like modifier-activating enzyme ATG7 N-terminus

Gene Ontology

Mouse over links to read term descriptions.
GO:0005737   cytoplasm  
GO:0008641   small protein activating enzyme activity  
GO:0005488   binding  
GO:0003824   catalytic activity  
GO:0008152   metabolic process  
GO:0006914   autophagy  

Metabolic Pathways

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
902 1191 1zud (1) 1 244 24.00 0 1 0.22 -0.39
920 1229 1ngv (B) 39 435 15.00 0 1 -0.15 0.22
104 249 3t7h (A) 5 107 33.00 0 0.14 -0.26979 1.77
429 495 5d2q (A) 116 182 10.00 0 0 0.4099 -2.86
461 638 5cwq (A) 4 179 26.00 0.0034 0.7 0.463934 -0.99
848 1295 4gsl (A) 255 608 42.00 0 1 0.453436 0.89
917 1029 4d79 (A) 18 119 22.00 0.0076 0.41 0.261935 -0.4
927 1062 1jw9 (B) 29 131 42.00 0.0000018 1 0.286402 0.07
1243 1330 4wjm (A) 125 205 36.00 0.082 0.6 0.415366 -0.18

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Tachyzoite, ME49 merozoite. Gregory Hehl AB
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_128264)

Species Accession Gene Product
Arabidopsis thaliana AT5G45900   ubiquitin-like modifier-activating enzyme atg7
Babesia bovis BBOV_IV008170   hypothetical protein
Brugia malayi Bm1_39695   E1-like protein-activating enzyme Gsa7p/Apg7p containing protein
Candida albicans CaO19.707   similar to S. cerevisiae ATG7 (YHR171W) protein-conjugating enzyme of the Apg12p-Apg5p conjugation pathway of autophagy
Candida albicans CaO19.8326   similar to S. cerevisiae ATG7 (YHR171W) protein-conjugating enzyme of the Apg12p-Apg5p conjugation pathway of autophagy
Caenorhabditis elegans CELE_M7.5   Protein ATG-7
Cryptosporidium hominis Chro.40017   hypothetical protein
Cryptosporidium parvum cgd4_40   APG7-like ubiquitin activating enzyme E1
Dictyostelium discoideum DDB_G0271096   E1-like enzyme family protein
Drosophila melanogaster Dmel_CG5489   Autophagy-specific gene 7
Echinococcus granulosus EgrG_001055800   ubiquitin modifier activating enzyme ATG7
Entamoeba histolytica EHI_064700   autophagy protein apg7, putative
Echinococcus multilocularis EmuJ_001055800   ubiquitin modifier activating enzyme ATG7
Homo sapiens ENSG00000197548   autophagy related 7
Leishmania braziliensis LbrM.07.0010   ubiquitin activating E1 enzyme, putative
Leishmania donovani LdBPK_070010.1   Ubiquitin-like modifier-activating enzyme ATG7, putative
Leishmania infantum LinJ.07.0010   ubiquitin activating E1 enzyme, putative
Leishmania major LmjF.07.0010   ubiquitin activating E1 enzyme, putative
Leishmania mexicana LmxM.07.0010   ubiquitin activating E1 enzyme, putative
Loa Loa (eye worm) LOAG_08126   hypothetical protein
Mus musculus ENSMUSG00000030314   autophagy related 7
Neospora caninum NCLIV_030510   thiF family domain-containing protein, putative
Oryza sativa 4326644   Os01g0614900
Plasmodium berghei PBANKA_0922200   autophagy-related protein 7, putative
Plasmodium falciparum PF3D7_1126100   autophagy-related protein 7, putative
Plasmodium knowlesi PKNH_0924000   autophagy-related protein 7, putative
Plasmodium vivax PVX_091922   autophagy-related protein 7, putative
Plasmodium yoelii PY06467   ubiquitin activating enzyme E1-like protein-related
Saccharomyces cerevisiae YHR171W   Atg7p
Schistosoma japonicum Sjp_0102790   ko:K08337 autophagy-related protein 7, putative
Schistosoma japonicum Sjp_0303750   ko:K08337 autophagy-related protein 7, putative
Schmidtea mediterranea mk4.005587.01  
Trypanosoma brucei gambiense Tbg972.10.13510   ubiquitin activating E1 enzyme, putative,autophagy protein, putative
Trypanosoma brucei Tb927.10.11180   Ubiquitin-like modifier-activating enzyme ATG7, putative
Trypanosoma congolense TcIL3000_10_9420   Ubiquitin-like modifier-activating enzyme ATG7, putative
Trypanosoma cruzi TcCLB.509641.30   Ubiquitin-like modifier-activating enzyme ATG7, putative
Trypanosoma cruzi TcCLB.507711.150   Ubiquitin-like modifier-activating enzyme ATG7, putative
Toxoplasma gondii TGME49_229690   autophagy-related protein 7 atg7, putative
Theileria parva TP01_0958   hypothetical protein
Trichomonas vaginalis TVAG_092470   autophagy protein, putative

Essentiality

TGME49_229690 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.11180 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.11180 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.11180 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.11180 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_229690 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens autophagy related 7 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model

Ranking Plot

No enough druggable targets predicted through repurposing network model to make a plot

Putative Drugs List


Compound Raw Global Species
0.0048 0.4768 0.0986

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier TGME49_229690 (Toxoplasma gondii), autophagy-related protein 7 atg7, putative
Title for this comment
Comment