Detailed view for LmjF.35.1750

Basic information

TDR Targets ID: 26204
Leishmania major, dynein-associated roadblock protein-like protein
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.8092 | Length (AA): 119 | MW (Da): 13517 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03259   Roadblock/LC7 domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005868   cytoplasmic dynein complex  
GO:0007018   microtubule-based movement  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
23 119 2b95 (A) 11 106 59.00 0 1 1.59 -1.96
11 119 1y4o (A) 1 104 56.00 0 1 1.64017 -1.13
30 116 3l7h (A) 9 94 64.00 0 1 1.63959 -1.87

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile amastigotes. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_128122)

Species Accession Gene Product
Brugia malayi Bm1_24930   dynein light chain 2B, cytoplasmic
Caenorhabditis elegans CELE_T24H10.6   Protein DYRB-1
Cryptosporidium hominis Chro.50229   hypothetical protein
Cryptosporidium parvum cgd5_1550   conserved hypothetical protein
Dictyostelium discoideum DDB_G0285855   cytoplasmic dynein light chain
Drosophila melanogaster Dmel_CG15171   CG15171 gene product from transcript CG15171-RA
Drosophila melanogaster Dmel_CG10751   roadblock
Echinococcus granulosus EgrG_001043700   dynein light chain
Echinococcus granulosus EgrG_001111200   dynein light chain
Echinococcus granulosus EgrG_001111300   dynein light chain
Echinococcus multilocularis EmuJ_001111300   dynein light chain
Echinococcus multilocularis EmuJ_001043700   dynein light chain
Echinococcus multilocularis EmuJ_001111200   dynein light chain
Giardia lamblia GL50803_14270   Dynein light chain
Homo sapiens ENSG00000168589   dynein, light chain, roadblock-type 2
Homo sapiens ENSG00000125971   dynein, light chain, roadblock-type 1
Leishmania braziliensis LbrM.34.1650   dynein-associated roadblock protein-like protein
Leishmania braziliensis LbrM.18.1070   dynein light chain 2B, cytoplasmic, putative
Leishmania donovani LdBPK_181010.1   Outer arm dynein light chain 7
Leishmania donovani LdBPK_351740.1   dynein-associated roadblock protein-like protein
Leishmania infantum LinJ.18.1010   dynein light chain 2B, cytoplasmic, putative
Leishmania infantum LinJ.35.1740   dynein-associated roadblock protein-like protein
Leishmania major LmjF.18.1010   dynein light chain 2B, cytoplasmic, putative
Leishmania major LmjF.35.1750   dynein-associated roadblock protein-like protein
Leishmania mexicana LmxM.18.1010   dynein light chain 2B, cytoplasmic, putative
Leishmania mexicana LmxM.34.1750   dynein-associated roadblock protein-like protein
Loa Loa (eye worm) LOAG_01797   dynein light chain 2B
Mus musculus ENSMUSG00000034467   dynein light chain roadblock-type 2
Mus musculus ENSMUSG00000047459   dynein light chain roadblock-type 1
Neospora caninum NCLIV_025350   hypothetical protein
Plasmodium berghei PBANKA_0504400   conserved Plasmodium protein, unknown function
Plasmodium berghei PBANKA_0504300   flagellar outer arm dynein-associated protein, putative
Plasmodium falciparum PF3D7_1020100   flagellar outer arm dynein-associated protein, putative
Plasmodium falciparum PF3D7_1020200   conserved Plasmodium protein, unknown function
Plasmodium knowlesi PKNH_0604400   conserved Plasmodium protein, unknown function
Plasmodium knowlesi PKNH_0604300   roadblock/LC7 family member, putative
Plasmodium vivax PVX_001868   hypothetical protein, conserved
Plasmodium vivax PVX_001872   hypothetical protein, conserved
Plasmodium yoelii PY00604   roadblock
Plasmodium yoelii PY00603   hypothetical protein
Schistosoma japonicum Sjp_0076890   ko:K10419 dynein light chain roadblock-type, putative
Schistosoma mansoni Smp_056780   dynein light chain
Schmidtea mediterranea mk4.017572.00  
Schmidtea mediterranea mk4.002498.02   Dynein light chain, putative
Schmidtea mediterranea mk4.000280.07   Dynein light chain roadblock-type 1
Schmidtea mediterranea mk4.000879.01   Dynein light chain, putative
Schmidtea mediterranea mk4.000421.03   Dynein light chain, putative
Trypanosoma brucei gambiense Tbg972.10.15860   dynein light chain 2B, cytoplasmic, putative,predicted dynein modulator,roadblock/LC7 family member
Trypanosoma brucei Tb927.9.14480   dynein-associated protein, putative
Trypanosoma brucei Tb927.10.13110   Outer arm dynein light chain 7
Trypanosoma cruzi TcCLB.510761.73   dynein-associated protein, putative
Trypanosoma cruzi TcCLB.503521.30   Outer arm dynein light chain 7
Trypanosoma cruzi TcCLB.506359.80   Dynein light chain roadblock-type
Toxoplasma gondii TGME49_262480   dynein light chain roadblock-type 2, putative
Trichomonas vaginalis TVAG_354740   dynein light chain, putative
Trichomonas vaginalis TVAG_050160   dynein light chain, putative
Trichomonas vaginalis TVAG_207270   dynein light chain, putative
Trichomonas vaginalis TVAG_116140   dynein light chain, putative

Essentiality

LmjF.35.1750 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.13110 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.13110 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.13110 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.13110 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb09.211.4920 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb09.211.4920 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.211.4920 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb09.211.4920 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_T24H10.6 Caenorhabditis elegans embryonic lethal wormbase
PBANKA_0504400 Plasmodium berghei Slow plasmo
TGME49_262480 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier LmjF.35.1750 (Leishmania major), dynein-associated roadblock protein-like protein
Title for this comment
Comment