Detailed view for TGME49_229180

Basic information

TDR Targets ID: 262073
Toxoplasma gondii, HEAT repeat-containing protein
Source Database / ID:  ToxoDB 

pI: 4.4261 | Length (AA): 1238 | MW (Da): 136164 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF13513   HEAT-like repeat

Gene Ontology

Mouse over links to read term descriptions.
GO:0008536   Ran GTPase binding  
GO:0005737   cytoplasm  
GO:0005643   nuclear pore  
GO:0005634   nucleus  
GO:0008565   protein transporter activity  
GO:0005488   binding  
GO:0006886   intracellular protein transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 1235 3w3w (A) 7 1086 22.00 0 1 0.994069 0.71
3 1235 3w3z (A) 9 1086 23.00 0 1 1.00826 0.56
293 335 4x8w (H) 1 302 56.00 0.021 0.18 0.268733 3.34
456 562 3vwa (A) 433 536 20.00 0 0.56 0.40873 -1.17
492 642 4u2x (D) 344 502 14.00 0 1 0.367271 -0.67

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Oocyst. Fritz HM
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_127537)

Species Accession Gene Product
Arabidopsis thaliana AT5G19820   protein EMBRYO DEFECTIVE 2734
Babesia bovis BBOV_IV004650   karyopherin beta, putative
Brugia malayi Bm1_33925   kap beta 3 protein
Candida albicans CaO19.5085   karyopherin
Candida albicans CaO19.12551   karyopherin
Caenorhabditis elegans CELE_C53D5.6   Protein IMB-3
Cryptosporidium hominis Chro.70365   karyopherin beta
Cryptosporidium parvum cgd7_3270   importin/karyopherin (Arm repeats)
Dictyostelium discoideum DDB_G0291650   importin subunit beta-3
Drosophila melanogaster Dmel_CG1059   Karyopherin beta 3
Echinococcus granulosus EgrG_000063800   importin 5
Entamoeba histolytica EHI_081250   importin beta-3 family protein
Entamoeba histolytica EHI_151640   HEAT repeat domain containing protein
Entamoeba histolytica EHI_064450   HEAT repeat domain containing protein
Echinococcus multilocularis EmuJ_000063800   importin 5
Giardia lamblia GL50803_15106   Importin beta-3 subunit
Homo sapiens ENSG00000065150   importin 5
Homo sapiens ENSG00000137040   RAN binding protein 6
Leishmania braziliensis LbrM.32.2360   hypothetical protein, conserved
Leishmania donovani LdBPK_322280.1   hypothetical protein, conserved
Leishmania infantum LinJ.32.2280   hypothetical protein, conserved
Leishmania major LmjF.32.2150   hypothetical protein, conserved
Leishmania mexicana LmxM.31.2150   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_01323   kap beta 3 protein
Mus musculus ENSMUSG00000074909   RAN binding protein 6
Mus musculus ENSMUSG00000030662   importin 5
Neospora caninum NCLIV_030120   importin beta-3 subunit, putative
Oryza sativa 4333825   Os03g0701000
Oryza sativa 4343696   Os07g0575100
Plasmodium berghei PBANKA_1238800   karyopherin beta, putative
Plasmodium falciparum PF3D7_0524000   karyopherin beta
Plasmodium knowlesi PKNH_1008900   karyopherin beta, putative
Plasmodium vivax PVX_080050   karyopherin beta, putative
Plasmodium yoelii PY00155   karyopherin beta
Saccharomyces cerevisiae YMR308C   Pse1p
Schistosoma japonicum Sjp_0051530   Importin subunit beta-3, putative
Schistosoma japonicum Sjp_0051540   Importin subunit beta-3, putative
Schistosoma mansoni Smp_032510   importin-beta 3
Schmidtea mediterranea mk4.008618.02  
Schmidtea mediterranea mk4.011332.01  
Schmidtea mediterranea mk4.020852.00  
Schmidtea mediterranea mk4.011332.02   Putative importin-beta 3
Trypanosoma brucei gambiense Tbg972.11.17250   hypothetical protein, conserved
Trypanosoma brucei Tb927.11.15370   Importin subunit beta-4, putative
Trypanosoma congolense TcIL3000.11.15520   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.511707.39   Importin subunit beta-4, putative
Trypanosoma cruzi TcCLB.506425.40   Importin subunit beta-4, putative
Toxoplasma gondii TGME49_229180   HEAT repeat-containing protein
Theileria parva TP01_0283   karyopherin beta, putative
Trichomonas vaginalis TVAG_405250   importin beta-3, putative
Trichomonas vaginalis TVAG_250580   conserved hypothetical protein
Trichomonas vaginalis TVAG_161220   importin beta-3, putative

Essentiality

TGME49_229180 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.7010 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.01.7010 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.7010 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.7010 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C53D5.6 Caenorhabditis elegans embryonic lethal wormbase
CELE_C53D5.6 Caenorhabditis elegans larval arrest wormbase
CELE_C53D5.6 Caenorhabditis elegans slow growth wormbase
CELE_C53D5.6 Caenorhabditis elegans sterile wormbase
YMR308C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1238800 Plasmodium berghei Dispensable plasmo
TGME49_229180 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier TGME49_229180 (Toxoplasma gondii), HEAT repeat-containing protein
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