Detailed view for TGME49_249010

Basic information

TDR Targets ID: 262093
Toxoplasma gondii, hypothetical protein
Source Database / ID:  ToxoDB 

pI: 5.4382 | Length (AA): 540 | MW (Da): 59102 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF13649   Methyltransferase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0008168   methyltransferase activity  
GO:0008152   metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
174 424 3bus (A) 35 270 17.00 0 0.96 0.579515 0.22
177 257 1nv8 (A) 102 182 17.00 0.00033 0.16 0.2988 0.22
184 279 5bxy (A) 7 106 16.00 0 0.31 0.333478 -0.26
200 264 1ri5 (A) 68 126 42.00 0.19 0.41 0.30467 1.42

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 merozoite. Gregory Hehl AB
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Show/Hide expression data references
  • Gregory ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_129355)

Species Accession Gene Product
Arabidopsis thaliana AT1G66680   putative S locus-linked protein
Brugia malayi Bm1_28635   Hypothetical 28.7 kDa protein in RNR3-ARC15 intergenic region
Brugia malayi Bm1_06970   Hypothetical 28.7 kDa protein in RNR3-ARC15 intergenic region
Candida albicans CaO19.11066   predicted methyltransferase protein
Candida albicans CaO19.3582   predicted methyltransferase protein
Caenorhabditis elegans CELE_F29B9.1   Protein F29B9.1
Cryptosporidium hominis Chro.50409   CG9643-PA
Cryptosporidium parvum cgd5_4330   conserved methylase
Dictyostelium discoideum DDB_G0278427   hypothetical protein
Drosophila melanogaster Dmel_CG9643   CG9643 gene product from transcript CG9643-RB
Echinococcus granulosus EgrG_000832800   methyltransferase protein 10
Entamoeba histolytica EHI_110190   hypothetical protein, conserved
Echinococcus multilocularis EmuJ_000832800   methyltransferase protein 10
Homo sapiens 399818   methyltransferase like 10
Loa Loa (eye worm) LOAG_09862   hypothetical protein
Mus musculus ENSMUSG00000030960   methyltransferase like 10
Neospora caninum NCLIV_065110   hypothetical protein, conserved
Oryza sativa 4324111   Os01g0686400
Oryza sativa 4326040   Os01g0121100
Onchocerca volvulus OVOC9301  
Plasmodium berghei PBANKA_0817600   methyltransferase, putative
Plasmodium falciparum PF3D7_0916600   methyltransferase, putative
Plasmodium knowlesi PKNH_0714600   methyltransferase, putative
Plasmodium vivax PVX_099255   methyltransferase, putative
Plasmodium yoelii PY06964   Drosophila melanogaster AT11165p-related
Saccharomyces cerevisiae YIL064W   See1p
Schmidtea mediterranea mk4.006166.00   Methyltransferase-like protein 10
Schmidtea mediterranea mk4.006038.00   Methyltransferase-like protein 10
Toxoplasma gondii TGME49_249010   hypothetical protein

Essentiality

TGME49_249010 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
PBANKA_0817600 Plasmodium berghei Dispensable plasmo
TGME49_249010 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_249010 (Toxoplasma gondii), hypothetical protein
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