Detailed view for TGME49_207070

Basic information

TDR Targets ID: 262103
Toxoplasma gondii, glycosyl transferase, putative
Source Database / ID:  ToxoDB 

pI: 10.7011 | Length (AA): 358 | MW (Da): 39462 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 2

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF08660   Oligosaccharide biosynthesis protein Alg14 like

Gene Ontology

Mouse over links to read term descriptions.
GO:0006488   dolichol-linked oligosaccharide biosynthetic process  

Metabolic Pathways

N-Glycan biosynthesis (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
117 241 4kv7 (A) 218 370 29.00 0.81 0.7 0.324462 0.09
203 235 5bw9 (n) 33 87 56.00 0.11 0.08 0.766479 -1.42

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Bradyzoite. Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst. Gregory Hehl AB Fritz HM
Show/Hide expression data references
  • Gregory ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_128444)

Species Accession Gene Product
Arabidopsis thaliana AT4G18230   hypothetical protein
Babesia bovis BBOV_IV004090   conserved membrane protein, putative
Brugia malayi Bm1_52490   cap8F
Candida albicans CaO19.12823   similar to S. cerevisiae YBR070C
Candida albicans CaO19.5363   similar to S. cerevisiae YBR070C
Caenorhabditis elegans CELE_M02B7.4   Protein HPO-16
Cryptosporidium hominis Chro.70549   hypothetical protein
Cryptosporidium parvum cgd7_4930   secreted glycosyltransferase, possible transmembrane domain near C-terminus
Dictyostelium discoideum DDB_G0290339   hypothetical protein
Drosophila melanogaster Dmel_CG6308   CG6308 gene product from transcript CG6308-RA
Echinococcus granulosus EgrG_000865200   UDP N acetylglucosamine transferase subunit
Entamoeba histolytica EHI_049660   hypothetical protein, conserved
Echinococcus multilocularis EmuJ_000865200   UDP N acetylglucosamine transferase subunit
Homo sapiens 199857   ALG14, UDP-N-acetylglucosaminyltransferase subunit
Leishmania braziliensis LbrM.30.0610   glycosyltransferase family 28 protein, putative
Leishmania donovani LdBPK_300550.1   glycosyltransferase family 28 protein, putative
Leishmania infantum LinJ.30.0550   glycosyltransferase family 28 protein, putative
Leishmania major LmjF.30.0530   glycosyltransferase family 28 protein, putative
Leishmania mexicana LmxM.29.0530   glycosyltransferase family 28 protein, putative
Mus musculus ENSMUSG00000039887   asparagine-linked glycosylation 14
Neospora caninum NCLIV_052990   Glycosyltransferase family protein, related
Oryza sativa 4333143   Os03g0423200
Plasmodium berghei PBANKA_0308400   UDP-N-acetylglucosamine transferase subunit ALG14, putative
Plasmodium falciparum PF3D7_0211600   UDP-N-acetylglucosamine transferase subunit ALG14, putative
Plasmodium knowlesi PKNH_0409600   UDP-N-acetylglucosamine transferase subunit ALG14, putative
Plasmodium vivax PVX_002950   hypothetical protein, conserved
Plasmodium yoelii PY04598   glycosyl transferase
Saccharomyces cerevisiae YBR070C   N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase anchoring subunit ALG14
Schistosoma japonicum Sjp_0094960   ko:K07441 beta-1,4-N-acetylglucosaminyltransferase, putative
Schistosoma mansoni Smp_045430.1   glycosyltransferase-related
Schistosoma mansoni Smp_045430.3   glycosyltransferase-related
Schmidtea mediterranea mk4.001668.05  
Trypanosoma brucei gambiense Tbg972.6.1680   glycosyltransferase family 28 protein, putative
Trypanosoma brucei Tb927.6.1960   glycosyltransferase family 28 protein, putative
Trypanosoma congolense TcIL3000_6_1540   glycosyltransferase family 28 protein, putative
Trypanosoma cruzi TcCLB.504071.90   glycosyltransferase family 28 protein, putative
Toxoplasma gondii TGME49_207070   glycosyl transferase, putative
Trichomonas vaginalis TVAG_038800   glycosyl transferase, putative

Essentiality

TGME49_207070 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.1960 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.6.1960 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.6.1960 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.6.1960 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
YBR070C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0308400 Plasmodium berghei Slow plasmo
TGME49_207070 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier TGME49_207070 (Toxoplasma gondii), glycosyl transferase, putative
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