Detailed view for TGME49_268840

Basic information

TDR Targets ID: 262204
Toxoplasma gondii, N6-adenosine-methyltransferase, putative
Source Database / ID:  ToxoDB 

pI: 6.3904 | Length (AA): 525 | MW (Da): 57853 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF05063   MT-A70

Gene Ontology

Mouse over links to read term descriptions.
GO:0008168   methyltransferase activity  
GO:0003676   nucleic acid binding  
GO:0032259   methylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
13 83 2pne (A) 14 81 21.00 0.56 0.26 0.235238 0.77
51 115 5ctd (C) 6 71 38.00 0.75 0.01 0.11551 1.64
198 515 5il2 (B) 117 402 51.00 0 1 1.10471 -0.28
198 482 5tey (B) 117 378 55.00 0 1 0.913557 0.14

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 merozoite. Hehl AB
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_130096)

Species Accession Gene Product
Arabidopsis thaliana AT4G09980   methyltransferase-like protein 1
Babesia bovis BBOV_IV005850   conserved hypothetical protein
Candida albicans CaO19.3736   Transcription factor involved in karyogamy.
Candida albicans CaO19.11221   transcription factor involved in karyogamy
Drosophila melanogaster Dmel_CG7818   CG7818 gene product from transcript CG7818-RA
Echinococcus granulosus EgrG_000931900   methyltransferase protein 14
Echinococcus multilocularis EmuJ_000931900   methyltransferase protein 14
Homo sapiens ENSG00000145388   methyltransferase like 14
Mus musculus ENSMUSG00000028114   methyltransferase like 14
Neospora caninum NCLIV_037510   mRNA (2-O-methyladenosine-N(6)-)- methyltransferase, putative
Oryza sativa 4348759   Os10g0447600
Oryza sativa 4324378   Os01g0267100
Oryza sativa 4331618   Os03g0147700
Plasmodium berghei PBANKA_1450100   mRNA methyltransferase, putative
Plasmodium falciparum PF3D7_1235500   mRNA methyltransferase, putative
Plasmodium knowlesi PKNH_1455200   methyltransferase, putative
Plasmodium vivax PVX_100725   hypothetical protein, conserved
Plasmodium yoelii PY00961   Unknown-related
Saccharomyces cerevisiae YCL055W   Kar4p
Schistosoma japonicum Sjp_0131550   ko:K05925 mRNA (2'-O-methyladenosine-N6-)-methyltransferase [EC2.1.1.62], putative
Schistosoma japonicum Sjp_0306420   ko:K08731 baculoviral IAP repeat-containing 5, putative
Schistosoma japonicum Sjp_0131570   Baculoviral IAP repeat-containing protein 5, putative
Schistosoma mansoni Smp_172190.1   hypothetical protein
Schistosoma mansoni Smp_172190.2   hypothetical protein
Schmidtea mediterranea mk4.009574.01  
Schmidtea mediterranea mk4.000430.11   N6-adenosine-methyltransferase subunit METTL14
Toxoplasma gondii TGME49_268840   N6-adenosine-methyltransferase, putative

Essentiality

TGME49_268840 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
PBANKA_1450100 Plasmodium berghei Essential plasmo
TGME49_268840 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.3

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_268840 (Toxoplasma gondii), N6-adenosine-methyltransferase, putative
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