Detailed view for TGME49_280400

Basic information

TDR Targets ID: 262213
Toxoplasma gondii, hypothetical protein
Source Database / ID:  ToxoDB 

pI: 8.9462 | Length (AA): 1157 | MW (Da): 130571 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 12

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF10034   Q-cell neuroblast polarisation

Gene Ontology

Mouse over links to read term descriptions.
GO:0016021   integral to membrane  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
631 737 4f35 (B) 122 223 33.00 0.32 0.36 0.167981 1.13
840 1022 2pl2 (A) 48 219 15.00 0.12 0.08 0.236768 -0.03
909 1008 5dse (A) 731 828 16.00 0.35 0.48 0.36403 -1.02
960 1053 2vyi (A) 108 199 17.00 0.5 0.19 0.292845 -0.55

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 merozoite. Gregory Hehl AB
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 Oocyst, ME49 Bradyzoite. Fritz HM Sibley/Greg
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_131282)

Species Accession Gene Product
Babesia bovis BBOV_II005590   hypothetical protein
Brugia malayi Bm1_46535   Protein dpy-19
Caenorhabditis elegans CELE_F22B7.10   Protein DPY-19
Cryptosporidium hominis Chro.40248   hypothetical protein
Cryptosporidium parvum cgd4_2180   hypothetical protein with 12+ transmembrane domains
Drosophila melanogaster Dmel_CG6659   CG6659 gene product from transcript CG6659-RA
Echinococcus granulosus EgrG_000553300   protein dpy 19 1
Echinococcus multilocularis EmuJ_000553300  
Homo sapiens 101928208   probable C-mannosyltransferase DPY19L2-like
Homo sapiens ENSG00000177990   dpy-19-like 2 (C. elegans)
Homo sapiens ENSG00000173852   dpy-19-like 1 (C. elegans)
Homo sapiens 102724565   probable C-mannosyltransferase DPY19L2-like
Loa Loa (eye worm) LOAG_04614   hypothetical protein
Mus musculus ENSMUSG00000043067   dpy-19-like 1 (C. elegans)
Mus musculus ENSMUSG00000085576   dpy-19-like 2 (C. elegans)
Neospora caninum NCLIV_019750   hypothetical protein, conserved
Plasmodium berghei PBANKA_1224500   C-mannosyltransferase, putative
Plasmodium falciparum PF3D7_0806200   C-mannosyltransferase, putative
Plasmodium knowlesi PKNH_0112700   C-mannosyltransferase, putative
Plasmodium vivax PVX_088240   hypothetical protein, conserved
Plasmodium yoelii PY04694   hypothetical protein
Schistosoma japonicum Sjp_0023600   Protein dpy-19 homolog 2, putative
Schistosoma mansoni Smp_123950   hypothetical protein
Schmidtea mediterranea mk4.001912.00   C-mannosyltransferase dpy-19 homolog
Schmidtea mediterranea mk4.001022.09   C-mannosyltransferase dpy-19
Toxoplasma gondii TGME49_280400   hypothetical protein

Essentiality

TGME49_280400 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
TGME49_280400 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_280400 (Toxoplasma gondii), hypothetical protein
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