Detailed view for TGME49_312310

Basic information

TDR Targets ID: 262360
Toxoplasma gondii, ATPase, AAA family protein
Source Database / ID:  ToxoDB 

pI: 5.9658 | Length (AA): 798 | MW (Da): 85481 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00004   ATPase family associated with various cellular activities (AAA)

Gene Ontology

Mouse over links to read term descriptions.
GO:0017111   nucleoside-triphosphatase activity  
GO:0005524   ATP binding  
GO:0000166   nucleotide binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
301 352 2wt7 (A) 147 193 51.00 0.98 0.05 0.439163 -0.16
409 782 4ko8 (A) 95 466 23.00 0 1 0.706072 0.32
507 796 3d8b (A) 390 671 44.00 0 1 0.861409 -0.65
518 798 4l15 (A) 316 587 38.00 0 1 0.82913 -0.54

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Hehl AB Fritz HM Sibley/Greg
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_127068)

Species Accession Gene Product
Arabidopsis thaliana AT2G45500   AAA-type ATPase-like protein
Arabidopsis thaliana AT3G27120   P-loop containing nucleoside triphosphate hydrolases superfamily protein
Babesia bovis BBOV_I000400   ATPase, AAA family domain containing protein
Brugia malayi Bm1_02420   ATPase, AAA family protein
Brugia malayi Bm1_53365   ATPase, AAA family protein
Candida albicans CaO19.7558   potential AAA family ATPase similar to S. cerevisiae SAP1 (YER047C)
Caenorhabditis elegans CELE_C24B5.2   Protein SPAS-1, isoform B
Caenorhabditis elegans CELE_F32D1.1   Protein FIGL-1
Cryptosporidium hominis Chro.50096   AAA family ATPase
Cryptosporidium parvum cgd5_2790   katanin p60/fidgetin family with AAA ATpase
Dictyostelium discoideum DDB_G0287165   AAA ATPase domain-containing protein
Dictyostelium discoideum DDB_G0268334   hypothetical protein
Drosophila melanogaster Dmel_CG3326   CG3326 gene product from transcript CG3326-RB
Drosophila melanogaster Dmel_CG5977   spastin
Echinococcus granulosus EgrG_001005200   fidgetin protein 1
Entamoeba histolytica EHI_036890   ATPase, Vps4 oligomerisation domain containing protein
Echinococcus multilocularis EmuJ_001005200   fidgetin protein 1
Homo sapiens ENSG00000021574   spastin
Homo sapiens ENSG00000132436   fidgetin-like 1
Leishmania braziliensis LbrM.15.0500   katanin-like protein,serine peptidase, Clan SJ, family S16, putative
Leishmania donovani LdBPK_150520.1   katanin-like protein
Leishmania infantum LinJ.15.0520   katanin-like protein,serine peptidase, Clan SJ, family S16, putative
Leishmania major LmjF.15.0500   katanin-like protein,serine peptidase, Clan SJ, family S16, putative
Leishmania mexicana LmxM.15.0500   katanin-like protein,serine peptidase, Clan SJ, family S16, putative
Loa Loa (eye worm) LOAG_05931   fidgetin protein
Loa Loa (eye worm) LOAG_04996   ATPase
Mus musculus ENSMUSG00000035455   fidgetin-like 1
Mus musculus ENSMUSG00000024068   spastin
Neospora caninum NCLIV_055880   ATPase, AAA family protein, related
Oryza sativa 4339999   Os06g0130000
Oryza sativa 9271031   Os12g0443800
Plasmodium berghei PBANKA_1005500   AAA family ATPase, putative
Plasmodium falciparum PF3D7_0407900   AAA family ATPase, putative
Plasmodium knowlesi PKNH_0305900   AAA family ATPase, putative
Plasmodium vivax PVX_000850   AAA family ATPase, putative
Plasmodium yoelii PY02232   ATPase, AAA family, putative
Saccharomyces cerevisiae YPL074W   putative AAA family ATPase YTA6
Saccharomyces cerevisiae YER047C   putative AAA family ATPase SAP1
Schistosoma japonicum Sjp_0049080   ko:K01509 adenosinetriphosphatase [EC3.6.1.3], putative
Schistosoma mansoni Smp_002810   fidgetin like-1
Schmidtea mediterranea mk4.010616.00  
Schmidtea mediterranea mk4.002854.04   Fidgetin-like protein 1
Schmidtea mediterranea mk4.010616.01   Fidgetin-like protein 1
Schmidtea mediterranea mk4.001181.02   Probable spastin homolog spas-1
Trypanosoma brucei gambiense Tbg972.3.1190   AAA ATPase, putative,Spastin, putative
Trypanosoma brucei gambiense Tbg972.5.2570   hypothetical protein, conserved,serine peptidase, Clan SJ, family S16, putative
Trypanosoma brucei Tb927.3.1440   Spastin, putative
Trypanosoma brucei Tb927.5.1870   metalloprotease, putative
Trypanosoma congolense TcIL3000_0_25690   Spastin, putative
Trypanosoma congolense TcIL3000_0_15540   Spastin, putative
Trypanosoma congolense TcIL3000_0_08200   metalloprotease, putative
Trypanosoma cruzi TcCLB.508533.30   metalloprotease, putative
Trypanosoma cruzi TcCLB.509437.50   Spastin, putative
Trypanosoma cruzi TcCLB.509233.70   AAA ATPase, putative
Toxoplasma gondii TGME49_302150   ATPase, AAA family protein
Toxoplasma gondii TGME49_312310   ATPase, AAA family protein
Theileria parva TP02_0917   AAA family ATPase, putative
Trichomonas vaginalis TVAG_339190   spastin, putative

Essentiality

TGME49_312310 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.1870 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.1870 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.5.1870 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.1870 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.3.1440 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.3.1440 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.3.1440 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.3.1440 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F32D1.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_F32D1.1 Caenorhabditis elegans slow growth wormbase
CELE_F32D1.1 Caenorhabditis elegans sterile wormbase
PBANKA_1005500 Plasmodium berghei Dispensable plasmo
TGME49_312310 this record Toxoplasma gondii Essentiality uncertain sidik
TGME49_302150 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TGME49_312310 (Toxoplasma gondii), ATPase, AAA family protein
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