Detailed view for TGME49_263150

Basic information

TDR Targets ID: 262534
Toxoplasma gondii, tetratricopeptide repeat-containing protein
Source Database / ID:  ToxoDB 

pI: 4.4628 | Length (AA): 415 | MW (Da): 45417 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF14559   Tetratricopeptide repeat

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  
GO:0005488   binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
15 119 1na0 (A) 7 114 15.00 0 0.98 0.57 -1.04
46 115 2avp (A) 1 68 15.00 0.00000000037 0.76 0.52 -1.59
28 141 4zlh (A) 203 306 34.00 0.57 0.53 0.423199 0.59
67 146 5fzs (A) 22 104 33.00 0.000027 1 0.675271 -1.18
340 398 2vq2 (A) 46 107 10.00 0.56 0.04 0.143369 0.34

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 merozoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Hehl AB Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Tachyzoite. Gregory
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_131100)

Species Accession Gene Product
Candida albicans CaO19.12428   Interacts with protein phosphatase 2C
Candida albicans CaO19.4963   Interacts with protein phosphatase 2C
Cryptosporidium hominis Chro.70300   SPBC16D10.01c
Cryptosporidium parvum cgd7_2640   TPR repeat protein
Dictyostelium discoideum DDB_G0291013   hypothetical protein
Echinococcus granulosus EgrG_000099300   TPR repeat containing protein YDR161W
Echinococcus multilocularis EmuJ_000099300   TPR repeat containing protein YDR161W
Leishmania braziliensis LbrM.34.4060   hypothetical protein, conserved
Leishmania donovani LdBPK_354130.1   hypothetical protein, conserved
Leishmania infantum LinJ.35.4130   hypothetical protein, conserved
Leishmania major LmjF.35.4070   hypothetical protein, conserved
Leishmania mexicana LmxM.34.4070   hypothetical protein, conserved
Neospora caninum NCLIV_024760   SPBC16D10.01c, related
Saccharomyces cerevisiae YDR161W   hypothetical protein
Schmidtea mediterranea mk4.000363.03  
Trypanosoma brucei gambiense Tbg972.9.6410   hypothetical protein, conserved
Trypanosoma brucei Tb927.9.10880   hypothetical protein, conserved
Trypanosoma congolense TcIL3000_9_4430   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.508461.200   hypothetical protein, conserved
Toxoplasma gondii TGME49_263150   tetratricopeptide repeat-containing protein

Essentiality

TGME49_263150 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.2230 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb09.211.2230 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.211.2230 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb09.211.2230 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_263150 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_263150 (Toxoplasma gondii), tetratricopeptide repeat-containing protein
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