Detailed view for TGME49_320570

Basic information

TDR Targets ID: 262837
Toxoplasma gondii, elongation factor Tu, putative
Source Database / ID:  ToxoDB 

pI: 5.8984 | Length (AA): 586 | MW (Da): 63550 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00009   Elongation factor Tu GTP binding domain
PF03143   Elongation factor Tu C-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005525   GTP binding  
GO:0003924   GTPase activity  

Metabolic Pathways

mRNA surveillance pathway (KEGG)

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
126 583 1r5b (A) 215 661 45.00 0 1 1.21 -0.09
145 584 1f60 (A) 3 441 39.00 0 1 1.19 -1.09
52 583 3mmp (A) 177 1392 24.00 0 1 0.89165 1.11
145 583 1r5b (A) 234 661 48.00 0 1 1.16495 -0.01
147 582 3vmf (A) 4 427 39.00 0 1 1.27063 -0.94
147 584 1f60 (A) 5 441 42.00 0 1 1.29404 -0.64
147 582 1jny (A) 4 427 40.00 0 1 1.22463 -0.58

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Oocyst. Fritz HM
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_127225)

Species Accession Gene Product
Arabidopsis thaliana AT1G18070   translation elongation factor EF1A/initiation factor IF2gamma family protein
Babesia bovis BBOV_II000640   translation elongation factor EF-1, subunit alpha protein, putative
Brugia malayi Bm1_24170   Elongation factor Tu C-terminal domain containing protein
Candida albicans CaO19.1378   translation release factor 3
Candida albicans CaO19.8958   translation release factor 3
Caenorhabditis elegans CELE_H19N07.1   Protein ERFA-3, isoform B
Cryptosporidium parvum cgd2_2070   translation elongation factor EF-1, subunit alpha, putative
Dictyostelium discoideum DDB_G0277919   hypothetical protein
Drosophila melanogaster Dmel_CG6382   Ef1alpha-like factor
Echinococcus granulosus EgrG_000136000   g1 to s phase transition protein
Entamoeba histolytica EHI_004230   guanine nucleotide regulatory protein, putative
Echinococcus multilocularis EmuJ_000136000   g1 to s phase transition protein
Giardia lamblia GL50803_17460   G1 to S phase transition protein 1, putative
Homo sapiens ENSG00000103342   G1 to S phase transition 1
Homo sapiens ENSG00000189369   G1 to S phase transition 2
Leishmania braziliensis LbrM.11.0970   eukaryotic release factor 3, putative
Leishmania donovani LdBPK_111160.1   eukaryotic release factor 3, putative
Leishmania infantum LinJ.11.1160   eukaryotic release factor 3, putative
Leishmania major LmjF.11.1170   eukaryotic release factor 3, putative
Leishmania mexicana LmxM.11.1170   eukaryotic release factor 3, putative
Loa Loa (eye worm) LOAG_04858   elongation factor Tu domain-containing protein
Mus musculus ENSMUSG00000071723   G1 to S phase transition 2
Mus musculus 14852   G1 to S phase transition 1
Neospora caninum NCLIV_009700   Elongation factor 1-alpha, related
Oryza sativa 4335338   Os04g0270100
Plasmodium berghei PBANKA_0924900   translation elongation factor EF-1, subunit alpha, putative
Plasmodium falciparum PF3D7_1123400   translation elongation factor EF-1, subunit alpha, putative
Plasmodium knowlesi PKNH_0921200   translation elongation factor EF-1, putative
Plasmodium vivax PVX_091785   translation elongation factor EF-1, subunit alpha, putative
Plasmodium yoelii PY04385   Elongation factor Tu family, putative
Saccharomyces cerevisiae YDR172W   translation termination factor GTPase eRF3
Schistosoma japonicum Sjp_0215560   ko:K03267 G1 to S phase transition protein, putative
Schistosoma japonicum Sjp_0128460   Eukaryotic peptide chain release factor GTP-binding subunit ERF3A, putative
Schistosoma mansoni Smp_005180   hypothetical protein
Schmidtea mediterranea mk4.000515.13  
Trypanosoma brucei gambiense Tbg972.11.6940   eukaryotic release factor 3, putative
Trypanosoma brucei Tb927.11.6160   eukaryotic release factor 3, putative
Trypanosoma congolense TcIL3000_0_08320   eukaryotic release factor 3, putative
Trypanosoma congolense TcIL3000.11.6690   eukaryotic release factor 3, putative
Trypanosoma cruzi TcCLB.506297.300   eukaryotic release factor 3, putative
Trypanosoma cruzi TcCLB.508041.60   eukaryotic release factor 3, putative
Toxoplasma gondii TGME49_320570   elongation factor Tu, putative
Theileria parva TP04_0514   translation elongation factor EF-1, subunit alpha, putative
Trichomonas vaginalis TVAG_133120   sulfate adenylyltransferase subunit, putative

Essentiality

TGME49_320570 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.02.4030 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.02.4030 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.02.4030 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.02.4030 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_H19N07.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_H19N07.1 Caenorhabditis elegans larval arrest wormbase
CELE_H19N07.1 Caenorhabditis elegans slow growth wormbase
CELE_H19N07.1 Caenorhabditis elegans sterile wormbase
YDR172W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0924900 Plasmodium berghei Essential plasmo
TGME49_320570 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Saccharomyces cerevisiae translation termination factor GTPase eRF3 Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Leishmania major elongation factor 1-alpha 449 aa 38.4% 438 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier TGME49_320570 (Toxoplasma gondii), elongation factor Tu, putative
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