Detailed view for TGME49_273350

Basic information

TDR Targets ID: 262867
Toxoplasma gondii, molybdopterin converting factor, subunit 2 protein
Source Database / ID:  ToxoDB 

pI: 6.0885 | Length (AA): 489 | MW (Da): 52759 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02391   MoaE protein

Gene Ontology

Mouse over links to read term descriptions.
GO:0006777   Mo-molybdopterin cofactor biosynthetic process  

Metabolic Pathways

Folate biosynthesis (KEGG)
Global map (KEGG)
Sulfur relay system (KEGG)

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
101 218 1nvj (B) 22 127 35.00 0 0.98 0.71 0.21
102 206 2mqa (A) 20 124 15.00 0 0.03 0.404924 -0.84
148 254 4zch (A) 16 125 16.00 0 0.06 0.384014 -0.27
279 418 5mpo (C) 42 169 32.00 0 1 0.646499 -0.62
297 420 2omd (A) 13 135 28.00 0 0.91 0.628079 -0.14

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 Oocyst. Fritz HM
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 merozoite. Hehl AB
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_128296)

Species Accession Gene Product
Arabidopsis thaliana AT2G43760   Molybdopterin synthase catalytic subunit
Caenorhabditis elegans CELE_T27A3.6   Protein T27A3.6
Dictyostelium discoideum DDB_G0271864   molybdenum cofactor synthesis protein 2 large subunit
Drosophila melanogaster Dmel_CG10238   Molybdenum cofactor synthesis 2
Escherichia coli b0785   molybdopterin synthase, large subunit
Homo sapiens ENSG00000164172   molybdenum cofactor synthesis 2
Loa Loa (eye worm) LOAG_00832   hypothetical protein
Mus musculus ENSMUSG00000015536   molybdenum cofactor synthesis 2
Mycobacterium tuberculosis Rv3119   Probable molybdenum cofactor biosynthesis protein E MoaE1 (molybdopterin converting factor large subunit) (molybdopterin [MPT] c
Mycobacterium tuberculosis Rv0866   Probable molybdenum cofactor biosynthesis protein E2 MoaE2 (molybdopterin converting factor large subunit) (molybdopterin [MPT]
Mycobacterium tuberculosis Rv3323c   Probable MoaD-MoaE fusion protein MoaX
Mycobacterium ulcerans MUL_0284   molybdenum cofactor biosynthesis protein E2 MoaE2
Oryza sativa 4328260   Os02g0140300
Onchocerca volvulus OVOC254   Molybdopterin synthase catalytic subunit homolog
Schistosoma japonicum Sjp_0306770   ko:K03635 molybdenum cofactor biosynthesis protein E, putative
Schmidtea mediterranea mk4.019893.00   Molybdopterin synthase catalytic subunit
Schmidtea mediterranea mk4.001953.02   Molybdopterin synthase catalytic subunit
Toxoplasma gondii TGME49_273350   molybdopterin converting factor, subunit 2 protein

Essentiality

TGME49_273350 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu882 Mycobacterium tuberculosis non-essential nmpdr
mtu3383 Mycobacterium tuberculosis non-essential nmpdr
b0785 Escherichia coli non-essential goodall
TGME49_273350 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_273350 (Toxoplasma gondii), molybdopterin converting factor, subunit 2 protein
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