Detailed view for TGME49_277760

Basic information

TDR Targets ID: 262917
Toxoplasma gondii, adenylosuccinate lyase, putative
Source Database / ID:  ToxoDB 

pI: 6.2478 | Length (AA): 661 | MW (Da): 74234 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF09418   Protein of unknown function (DUF2009)

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 Oocyst. Fritz HM
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Show/Hide expression data references
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_131912)

Species Accession Gene Product
Babesia bovis BBOV_III006820   hypothetical protein
Cryptosporidium hominis Chro.10179   hypothetical protein
Cryptosporidium parvum cgd1_1550   conserved hypothetical protein
Dictyostelium discoideum DDB_G0293552   hypothetical protein
Entamoeba histolytica EHI_148780   hypothetical protein, conserved
Leishmania braziliensis LbrM.34.4160   hypothetical protein, conserved
Leishmania donovani LdBPK_354230.1   Protein of unknown function (DUF2009), putative
Leishmania infantum LinJ.35.4230   hypothetical protein, conserved
Leishmania major LmjF.35.4160   hypothetical protein, conserved
Leishmania mexicana LmxM.34.4160   hypothetical protein, conserved
Neospora caninum NCLIV_068210   hypothetical protein, conserved
Plasmodium berghei PBANKA_1426100   conserved protein, unknown function
Plasmodium falciparum PF3D7_0811400   conserved protein, unknown function
Plasmodium knowlesi PKNH_1429300   conserved protein, unknown function
Plasmodium vivax PVX_123200   conserved protein, unknown function
Plasmodium yoelii PY04680   hypothetical protein
Trypanosoma brucei gambiense Tbg972.9.6280   hypothetical protein, conserved
Trypanosoma brucei Tb927.9.10690   Protein of unknown function (DUF2009), putative
Trypanosoma congolense TcIL3000_9_4310   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.508461.120   Protein of unknown function (DUF2009), putative
Toxoplasma gondii TGME49_277760   adenylosuccinate lyase, putative
Theileria parva TP04_0578   hypothetical protein, conserved

Essentiality

TGME49_277760 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.2100 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.211.2100 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb09.211.2100 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.211.2100 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1426100 Plasmodium berghei Dispensable plasmo
TGME49_277760 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_277760 (Toxoplasma gondii), adenylosuccinate lyase, putative
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