Detailed view for TGME49_309160

Basic information

TDR Targets ID: 262956
Toxoplasma gondii, IgA-specific metalloendopeptidase
Source Database / ID:  ToxoDB 

pI: 2.9745 | Length (AA): 847 | MW (Da): 92141 | Paralog Number: 1

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
383 562 5cwb (A) 8 179 28.00 0.089 0.92 0.535215 -0.78
452 611 5cwh (A) 3 160 11.00 0 0.01 0.237002 -0.99
489 597 1klp (A) 2 114 32.00 0.78 0.02 0.28139 0.32

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite, ME49 merozoite. Gregory Hehl AB
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile ME49 Oocyst. Fritz HM
Show/Hide expression data references
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_126854)

Species Accession Gene Product
Arabidopsis thaliana AT4G36520   chaperone DnaJ-domain containing protein
Babesia bovis BBOV_I002740   200 kDa antigen p200
Brugia malayi Bm1_05690   Low-density lipoprotein receptor domain class A containing protein
Brugia malayi Bm1_14520   Immunoglobulin I-set domain containing protein
Brugia malayi Bm1_32990   Apical junction molecule protein 1
Brugia malayi Bm1_17345   Low-density lipoprotein receptor domain class A containing protein
Candida albicans CaO19.14055   lysine/glutamic acid-rich protein
Candida albicans CaO19.6763   lysine/glutamic acid-rich protein
Caenorhabditis elegans CELE_H39E23.3   Protein H39E23.3
Caenorhabditis elegans CELE_F49E2.5   Protein F49E2.5, isoform E
Caenorhabditis elegans CELE_C25A11.4   Protein AJM-1, isoform E
Cryptosporidium parvum cgd4_770   Low complexity protein with large Glu repeat
Dictyostelium discoideum DDB_G0270864   hypothetical protein
Dictyostelium discoideum DDB_G0285911   LIM-type zinc finger-containing protein
Drosophila melanogaster Dmel_CG31551   CG31551 gene product from transcript CG31551-RA
Drosophila melanogaster Dmel_CG2839   CG2839 gene product from transcript CG2839-RA
Entamoeba histolytica EHI_059670   Rab family GTPase
Entamoeba histolytica EHI_050660   hypothetical protein, conserved
Entamoeba histolytica EHI_124620   hypothetical protein
Giardia lamblia GL50803_24122   Spindle pole protein, putative
Homo sapiens ENSG00000159450   trichohyalin
Homo sapiens ENSG00000100285   neurofilament, heavy polypeptide
Leishmania braziliensis LbrM.27.0260   kinetoplast-associated protein-like protein
Leishmania braziliensis LbrM.28.2410   glycoprotein 96-92, putative
Leishmania braziliensis LbrM.27.0250   kinetoplast-associated protein-like protein
Leishmania donovani LdBPK_282370.1   glycoprotein 96-92, putative
Leishmania infantum LinJ.28.2370   glycoprotein 96-92, putative
Leishmania infantum LinJ.27.0250   kinetoplast-associated protein-like protein
Leishmania major LmjF.27.0240   kinetoplast-associated protein-like protein
Loa Loa (eye worm) LOAG_06076   hypothetical protein
Loa Loa (eye worm) LOAG_01250   immunoglobulin I-set domain-containing protein
Loa Loa (eye worm) LOAG_15228   hypothetical protein
Loa Loa (eye worm) LOAG_06077   hypothetical protein
Loa Loa (eye worm) LOAG_10697   hypothetical protein
Loa Loa (eye worm) LOAG_05014   hypothetical protein
Loa Loa (eye worm) LOAG_10695   hypothetical protein
Mus musculus ENSMUSG00000052415   trichohyalin
Mus musculus ENSMUSG00000020396   neurofilament, heavy polypeptide
Neospora caninum NCLIV_015050   Proteophosphoglycan ppg4, related
Neospora caninum NCLIV_007540   hypothetical protein, conserved
Neospora caninum NCLIV_057110   IQ calmodulin-binding motif domain-containing protein, putative
Oryza sativa 4339717   Os05g0579900
Oryza sativa 4326602   Os01g0634300
Plasmodium berghei PBANKA_0901300   membrane associated erythrocyte binding-like protein
Plasmodium falciparum PF3D7_1147800   membrane associated erythrocyte binding-like protein
Plasmodium falciparum PF3D7_0504700   centrosomal protein CEP120, putative
Plasmodium knowlesi PKNH_0945700   membrane associated erythrocyte binding-like protein, putative
Plasmodium vivax PVX_097685   merozoite surface protein 3
Plasmodium vivax PVX_097690   merozoite surface protein 3
Plasmodium vivax PVX_097710   merozoite surface protein 3
Plasmodium vivax PVX_092975   merozoite adhesive erythrocytic binding protein
Plasmodium vivax PVX_097700   merozoite surface protein 3
Plasmodium vivax PVX_002510   Nucleosomal binding protein 1, putative
Plasmodium yoelii PY05977   erythrocyte binding protein
Schmidtea mediterranea mk4.000282.00  
Trypanosoma brucei gambiense Tbg972.7.8700   hypothetical protein, conserved
Trypanosoma brucei gambiense Tbg972.8.7560   kinetoplast-associated protein, putative
Trypanosoma brucei gambiense Tbg972.4.5305   hypothetical protein, conserved
Trypanosoma brucei Tb927.8.7260   kinetoplast-associated protein, putative
Trypanosoma brucei Tb927.4.5120   hypothetical protein, conserved
Trypanosoma brucei Tb927.7.7400   Basal body protein
Trypanosoma congolense TcIL3000_7_6020   hypothetical protein, conserved
Trypanosoma congolense TcIL3000_8_7490   hypothetical protein, conserved
Trypanosoma congolense TcIL3000_8_7380   kinetoplast-associated protein, putative
Trypanosoma cruzi TcCLB.510593.49   kinetoplast DNA-associated protein, putative
Trypanosoma cruzi TcCLB.510609.60   Basal body protein
Trypanosoma cruzi TcCLB.507969.10   vesicular transport-associated repeat protein, putative
Trypanosoma cruzi TcCLB.506303.80   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.506811.160   R27-2 protein, putative
Trypanosoma cruzi TcCLB.507895.124   hypothetical protein, conserved
Toxoplasma gondii TGME49_309160   IgA-specific metalloendopeptidase
Toxoplasma gondii TGME49_313900   non-specific serine/threonine protein kinase
Treponema pallidum TP0769   outer membrane protein (tmpB)
Theileria parva TP01_0576   hypothetical protein, conserved
Trichomonas vaginalis TVAG_413070   trichohyalin, putative
Trichomonas vaginalis TVAG_295230   hypothetical protein
Trichomonas vaginalis TVAG_168130   axoneme-associated protein mst101, putative
Trichomonas vaginalis TVAG_459620   conserved hypothetical protein
Trichomonas vaginalis TVAG_141490   Moesin, putative
Trichomonas vaginalis TVAG_399600   conserved hypothetical protein
Trichomonas vaginalis TVAG_028600   conserved hypothetical protein
Trichomonas vaginalis TVAG_354150   trichohyalin, putative
Trichomonas vaginalis TVAG_221970   trichohyalin, putative
Trichomonas vaginalis TVAG_368240   axoneme-associated protein mst101, putative
Trichomonas vaginalis TVAG_144410   trichohyalin, putative
Trichomonas vaginalis TVAG_210560   trichohyalin, putative
Trichomonas vaginalis TVAG_298320   trichohyalin, putative
Trichomonas vaginalis TVAG_398490   trichohyalin, putative
Trichomonas vaginalis TVAG_295240   hypothetical protein
Trichomonas vaginalis TVAG_005450   conserved hypothetical protein

Essentiality

TGME49_309160 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.5120 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.5120 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.5120 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.4.5120 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.8.7260 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.7260 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.8.7260 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.8.7260 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.7.7400 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.7.7400 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.7.7400 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.7.7400 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C25A11.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_C25A11.4 Caenorhabditis elegans slow growth wormbase
CELE_F49E2.5 Caenorhabditis elegans larval lethal wormbase
TGME49_313900 Toxoplasma gondii Essentiality uncertain sidik
TGME49_309160 this record Toxoplasma gondii Essentiality uncertain sidik
TGME49_313900 Toxoplasma gondii Probably non-essential sidik
TGME49_309160 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.3

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_309160 (Toxoplasma gondii), IgA-specific metalloendopeptidase
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