Detailed view for TGME49_222960

Basic information

TDR Targets ID: 263076
Toxoplasma gondii, SCY kinase-related protein (incomplete catalytic triad)
Source Database / ID:  ToxoDB 

pI: 6.9237 | Length (AA): 1065 | MW (Da): 112697 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0005488   binding  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 408 2b9h (A) 1 353 17.00 0 1 0.34 0.19
381 529 1lkj (A) 1 145 8.00 0.000063 0.06 0.2 -0.16
4 355 2j0j (A) 410 673 20.00 0 1 0.0908164 0.92
4 528 4mvf (A) 67 494 18.00 0 1 0.197258 1.48
7 367 3blh (A) 17 322 20.00 0 1 0.244267 0.57
20 529 5t6a (A) 58 503 16.00 0 1 0.350173 0.91
174 358 3p1a (A) 220 357 43.00 0.00028 0.79 0.0785089 1.37
412 618 3c5w (A) 15 571 16.00 0 1 0.419266 -0.58

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Hehl AB Fritz HM Sibley/Greg
Show/Hide expression data references
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_129163)

Species Accession Gene Product
Arabidopsis thaliana AT1G71410   putative protein kinase
Arabidopsis thaliana AT1G22870   protein kinase family protein
Brugia malayi Bm1_03320   Protein kinase domain containing protein
Candida albicans CaO19.765   similar to C terminus of S. cerevisiae SCY1 (YGL083W) suppressor of GTPase mutant
Candida albicans CaO19.764   similar to N terminus of S.cereviisae SCY1 (YGL083W) suppressor of GTPase mutant
Candida albicans CaO19.8384   similar to N terminus of S.cereviisae SCY1 (YGL083W) suppressor of GTPase mutant
Candida albicans CaO19.8385   similar to C terminus of S.cereviisae SCY1 (YGL083W) suppressor of GTPase mutant
Caenorhabditis elegans CELE_ZC581.9   Protein ZC581.9
Dictyostelium discoideum DDB_G0270808   SCY1 family protein kinase
Drosophila melanogaster Dmel_CG1951   CG1951 gene product from transcript CG1951-RA
Echinococcus granulosus EgrG_001185650   scy1 protein 2
Echinococcus multilocularis EmuJ_001185650   scy1 protein 2
Homo sapiens ENSG00000136021   SCY1-like 2 (S. cerevisiae)
Leishmania braziliensis LbrM.35.0210   hypothetical protein, unknown function
Leishmania donovani LdBPK_360150.1   hypothetical protein, unknown function
Leishmania infantum LinJ.36.0150   hypothetical protein, unknown function
Leishmania major LmjF.36.0140   hypothetical protein, unknown function
Leishmania mexicana LmxM.36.0140   hypothetical protein, unknown function
Loa Loa (eye worm) LOAG_07792   SCY1 protein kinase
Loa Loa (eye worm) LOAG_05285   hypothetical protein
Loa Loa (eye worm) LOAG_06308   SCY1 protein kinase
Mus musculus ENSMUSG00000069539   SCY1-like 2 (S. cerevisiae)
Neospora caninum NCLIV_005990   DEHA2D02640p, related
Oryza sativa 4326650   Os01g0616100
Saccharomyces cerevisiae YGL083W   Scy1p
Schistosoma japonicum Sjp_0100510   SCY1-like protein 2, putative
Schistosoma mansoni Smp_126740   protein kinase
Schmidtea mediterranea mk4.000982.12   SCY1-like protein 2
Schmidtea mediterranea mk4.000982.14   SCY1-like protein 2
Schmidtea mediterranea mk4.000982.13  
Schmidtea mediterranea mk4.010605.00  
Toxoplasma gondii TGME49_222960   SCY kinase-related protein (incomplete catalytic triad)
Trichomonas vaginalis TVAG_024740   serine-threonine protein kinase, putative
Trichomonas vaginalis TVAG_477100   TKL family protein kinase
Trichomonas vaginalis TVAG_052050   TKL family protein kinase
Trichomonas vaginalis TVAG_295330   conserved hypothetical protein
Trichomonas vaginalis TVAG_179950   serine-threonine protein kinase, putative

Essentiality

TGME49_222960 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
CELE_ZC581.9 Caenorhabditis elegans larval arrest wormbase
TGME49_222960 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 23.0% 309 aa Compounds References
Xenopus laevis Aurora kinase B-B 368 aa 20.4% 299 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0066 0.3101 0
0.0016 0.5 0.5
0.0067 0.5 0.5
0.0027 1 0.5
0.0036 0.5 0.5
0.0061 0.6883 0.6656
0.0039 0.5 0.5
0.0026 0.5 0.5
0.0092 1 0.5
0.0011 1 0.5
0.0029 0.5 0.5
0.0008 0.5 0.5
0.0039 0.9485 0.5
0.0098 0.3242 0.2614
0.0023 0.5 0.5
0.0007 0.5 0.5
0.0059 1 1
0.0062 0.6935 0
0.0091 1 0.5
0.0081 0.5 0.5
0.0004 0.5 0.5
0.0063 1 1
0.0033 0.5 0.5
0.0007 0.5 0.5
0.0018 0.5 0.5
0.0042 0.5 0.5
0.0059 1 1
0.0069 0.3067 1
0.0037 1 0.5
0.0039 0.5 0.5
0.0032 0.5 0.5
0.0012 0.5 0.5
0.0003 0.5 0.5
0.0012 0.5 0.5
0.0033 1 1
0.0016 0.5 0.5
0.0012 0.5 0.5
0.0007 0.5 0.5
0.0022 0.5 0.5
0.0059 1 1
0.0063 0.7244 0.7244
0.0064 0.3377 0
0.0056 1 0.5
0.0081 1 0.5
0.0088 0.4477 1
0.0032 0.5 0.5
0.0093 0.8828 0

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier TGME49_222960 (Toxoplasma gondii), SCY kinase-related protein (incomplete catalytic triad)
Title for this comment
Comment