Detailed view for LmjF.36.1360

Basic information

TDR Targets ID: 26309
Leishmania major, adenylate kinase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 9.0836 | Length (AA): 220 | MW (Da): 24869 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00406   Adenylate kinase

Gene Ontology

Mouse over links to read term descriptions.
GO:0019205   nucleobase, nucleoside, nucleotide kinase activity  
GO:0016776   phosphotransferase activity, phosphate group as acceptor  
GO:0006139   nucleobase, nucleoside, nucleotide and nucleic acid metabolic process  
GO:0019201   nucleotide kinase activity  
GO:0005524   ATP binding  
GO:0004017   adenylate kinase activity  

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 209 1zak (A) 5 213 42.00 0 1 1.57 -1.14
3 206 2eu8 (A) 1 214 31.00 0 1 1.34 -1.48
6 217 2bbw (A) 9 223 28.00 0 1 1.35 -0.75
2 218 1zak (A) 5 222 40.00 0.0000000024 1 1.52686 -0.58
2 206 4pzl (A) 0 217 33.00 0 1 1.38602 -1.2
2 218 2ak3 (A) 5 224 30.00 0 1 1.38056 -0.68
5 38 1q3t (A) 19 52 41.00 0.092 0.36 0.484045 1.1

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_138748)

Species Accession Gene Product
Arabidopsis thaliana AT5G35170   adenylate kinase family protein
Leishmania braziliensis LbrM.35.1480   adenylate kinase, putative
Leishmania donovani LdBPK_361410.1   adenylate kinase, putative
Leishmania infantum LinJ.36.1410   adenylate kinase, putative
Leishmania major LmjF.36.1360   adenylate kinase, putative
Leishmania mexicana LmxM.36.1360   adenylate kinase, putative
Oryza sativa 4345177   Os08g0288200
Trypanosoma brucei gambiense Tbg972.10.6990   adenylate kinase, putative
Trypanosoma brucei Tb927.10.5760   adenylate kinase, putative
Trypanosoma cruzi TcCLB.509733.180   adenylate kinase, putative

Essentiality

LmjF.36.1360 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.5760 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.5760 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.5760 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.5760 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Myokinase (2.7.4.3 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.
  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Leishmania donovani ( 1 )

Reagent availability

  • Reagent:
  • Target Type Source Notes
    LmjF.36.1360 purified protein BRENDA A protein with this EC number or name or sequence has been purified from Leishmania donovani ( 1 )

Bibliographic References

2 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.36.1360 (Leishmania major), adenylate kinase, putative
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