Detailed view for TGME49_248250

Basic information

TDR Targets ID: 263186
Toxoplasma gondii, translation initiation factor IF-2, putative
Source Database / ID:  ToxoDB 

pI: 8.3013 | Length (AA): 112 | MW (Da): 12567 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03660   PHF5-like protein

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

Spliceosome (KEGG)

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
3 92 5syb (A) 2 91 90.00 0 1 1.83137 -0.43

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 merozoite. Hehl AB
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_128251)

Species Accession Gene Product
Arabidopsis thaliana AT1G07170   splicing factor 3b-like protein
Arabidopsis thaliana AT2G30000   PHD finger-like domain-containing protein 5A
Brugia malayi Bm1_25020   PHD finger-like domain protein 5A
Candida albicans CaO19.9772   similar to S. cerevisiae YPR094W
Candida albicans CaO19.2230   similar to S. cerevisiae YPR094W
Caenorhabditis elegans CELE_Y54F10BM.14   Protein PHF-5
Cryptosporidium parvum cgd6_2400   hypothetical protein
Dictyostelium discoideum DDB_G0284403   PHD finger-like domain-containing protein 5A
Drosophila melanogaster Dmel_CG9548   CG9548 gene product from transcript CG9548-RA
Echinococcus granulosus EgrG_000615900   PHD finger 5A
Entamoeba histolytica EHI_081770   hypothetical protein, conserved
Echinococcus multilocularis EmuJ_000615900   PHD finger 5A
Giardia lamblia GL50803_32531   Hypothetical protein
Homo sapiens ENSG00000100410   PHD finger protein 5A
Leishmania braziliensis LbrM.35.3140   hypothetical protein, conserved
Leishmania donovani LdBPK_363070.1   PHF5-like protein, putative
Leishmania infantum LinJ.36.3070   hypothetical protein, conserved
Leishmania major LmjF.36.2920   hypothetical protein, conserved
Leishmania mexicana LmxM.36.2920   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_06442   hypothetical protein
Mus musculus 68479   PHD finger protein 5A
Neospora caninum NCLIV_064230   pre-mRNA-splicing factor, putative
Oryza sativa 4337303   Os04g0663300
Oryza sativa 4338563   Os05g0367000
Plasmodium berghei PBANKA_0502700   PHF5-like protein, putative
Plasmodium falciparum PF3D7_1018500   PHF5-like protein, putative
Plasmodium knowlesi PKNH_0602800   PHF5-like protein, putative
Plasmodium vivax PVX_001785   hypothetical protein, conserved
Saccharomyces cerevisiae YPR094W   Rds3p
Schistosoma japonicum Sjp_0058950   PHD finger-like domain-containing protein 5A, putative
Schistosoma mansoni Smp_011720   hypothetical protein
Schmidtea mediterranea mk4.001325.02   PHF-5
Trypanosoma brucei gambiense Tbg972.10.9060   hypothetical protein, conserved
Trypanosoma brucei Tb927.10.7390   PHF5-like protein, putative
Trypanosoma cruzi TcCLB.507681.60   PHF5-like protein, putative
Toxoplasma gondii TGME49_248250   translation initiation factor IF-2, putative
Theileria parva TP02_0180   hypothetical protein
Trichomonas vaginalis TVAG_447830   pre-mRNA-splicing factor ini1, putative

Essentiality

TGME49_248250 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.7390 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.7390 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.7390 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.7390 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y54F10BM.14 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y54F10BM.14 Caenorhabditis elegans larval lethal wormbase
YPR094W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0502700 Plasmodium berghei Essential plasmo
TGME49_248250 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier TGME49_248250 (Toxoplasma gondii), translation initiation factor IF-2, putative
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